Application | Comment | Organism |
---|---|---|
food industry | useful in starch industries. The addition of the enzyme in gelatinized rice and maize starches significantly increases the resistant starch type 3 yields (RS3). RS3 is of great importance for nutritionists and food industry, due to its reduced levels of plasma glucose and insulin, increased faecal bulk, and short-chain fatty acid production through fermentation in the large intestine | Paenibacillus barengoltzii |
Cloned (Comment) | Organism |
---|---|
heterologously expressed in Escherichia coli as an intracellular soluble protein | Paenibacillus barengoltzii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, 84% loss of activity | Paenibacillus barengoltzii | |
Cu2+ | 1 mM, 82% loss of activity | Paenibacillus barengoltzii | |
Hg2+ | 1 mM, 99% loss of activity | Paenibacillus barengoltzii | |
Sn2+ | 1 mM, 87% loss of activity | Paenibacillus barengoltzii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 1.6fold activation | Paenibacillus barengoltzii | |
Cr2+ | 1 mM, 1.1fold activation | Paenibacillus barengoltzii | |
Fe3+ | 1 mM, 1.4fold activation | Paenibacillus barengoltzii | |
K+ | 1 mM, 1.2fold activation | Paenibacillus barengoltzii | |
Mg2+ | 1 mM, 1.1fold activation | Paenibacillus barengoltzii | |
Ni2+ | 1 mM, 1.1fold activation | Paenibacillus barengoltzii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
SDS-PAGE | Paenibacillus barengoltzii |
75093 | - |
calculated from sequence | Paenibacillus barengoltzii |
78000 | - |
gel filtration | Paenibacillus barengoltzii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus barengoltzii | A0A0C5GWS2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Paenibacillus barengoltzii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | 12% of the activity compared to pullulan | Paenibacillus barengoltzii | ? | - |
? | |
pullulan + H2O | the enzyme exhibits strict substrate specificity towards pullulan, but shows relatively low activity towards amylopectin and no activity towards other tested polysaccharides | Paenibacillus barengoltzii | maltotriose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 75000, SDS-PAGE | Paenibacillus barengoltzii |
Synonyms | Comment | Organism |
---|---|---|
PbPulA | - |
Paenibacillus barengoltzii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Paenibacillus barengoltzii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 55 | 40°C: about 45% of maximal activity, 55°C: about 30% of maximal activity | Paenibacillus barengoltzii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
thermal denaturing half-lives: 501 min | Paenibacillus barengoltzii |
45 | - |
thermal denaturing half-lives: 253 min | Paenibacillus barengoltzii |
50 | - |
thermal denaturing half-lives: 33 min | Paenibacillus barengoltzii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
pullulan | Km-value: 2.94 mg/ml, pH 5.5, 50°C | Paenibacillus barengoltzii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Paenibacillus barengoltzii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.5 | 30 min, enzyme retains more than 80% of its activity | Paenibacillus barengoltzii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Paenibacillus barengoltzii | calculated from sequence | - |
5.22 |