Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) pLysS cells | Thermoanaerobacterium saccharolyticum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacterium saccharolyticum | C5IGQ7 | - |
- |
Thermoanaerobacterium saccharolyticum NTOU1 | C5IGQ7 | - |
- |
Purification (Comment) | Organism |
---|---|
BD TALON spin column chromatography, gel filtration | Thermoanaerobacterium saccharolyticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Thermoanaerobacterium saccharolyticum | maltotriose + maltose + ? | - |
? | |
amylopectin + H2O | - |
Thermoanaerobacterium saccharolyticum NTOU1 | maltotriose + maltose + ? | - |
? | |
amylose + H2O | - |
Thermoanaerobacterium saccharolyticum | maltotriose + maltose | - |
? | |
amylose + H2O | - |
Thermoanaerobacterium saccharolyticum NTOU1 | maltotriose + maltose | - |
? | |
glycogen + H2O | - |
Thermoanaerobacterium saccharolyticum | maltotriose + maltose | - |
? | |
additional information | the enzyme cleaves both alpha-1,4- and alpha-1,6-glycosidic linkages in starch, pullulan, amylopectin, and other related oligosaccharides | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
additional information | the enzyme cleaves both alpha-1,4- and alpha-1,6-glycosidic linkages in starch, pullulan, amylopectin, and other related oligosaccharides | Thermoanaerobacterium saccharolyticum NTOU1 | ? | - |
? | |
pullulan + H2O | - |
Thermoanaerobacterium saccharolyticum | maltotriose + ? | - |
? | |
pullulan + H2O | - |
Thermoanaerobacterium saccharolyticum NTOU1 | maltotriose + ? | - |
? | |
soluble starch + H2O | - |
Thermoanaerobacterium saccharolyticum | maltotriose + maltose | - |
? | |
soluble starch + H2O | - |
Thermoanaerobacterium saccharolyticum NTOU1 | maltotriose + maltose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
amylopullulanase | - |
Thermoanaerobacterium saccharolyticum |
Apu | - |
Thermoanaerobacterium saccharolyticum |
ApuK885 | truncated enzyme form | Thermoanaerobacterium saccharolyticum |
ApuM957 | truncated enzyme form | Thermoanaerobacterium saccharolyticum |
pullulanase type II | - |
Thermoanaerobacterium saccharolyticum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermoanaerobacterium saccharolyticum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
574.7 | - |
soluble starch | truncated enzyme form ApuK855, at pH 6.0 and 70°C | Thermoanaerobacterium saccharolyticum | |
877.9 | - |
soluble starch | truncated enzyme form ApuM957, at pH 6.0 and 70°C | Thermoanaerobacterium saccharolyticum | |
1751 | - |
pullulan | truncated enzyme form ApuM957, at pH 5.0 and 70°C | Thermoanaerobacterium saccharolyticum | |
1828 | - |
pullulan | truncated enzyme form ApuK855, at pH 5.0 and 70°C | Thermoanaerobacterium saccharolyticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
optimum pH for pullulanase activity | Thermoanaerobacterium saccharolyticum |
6 | - |
optimum pH for amylase activity | Thermoanaerobacterium saccharolyticum |