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Literature summary for 3.2.1.41 extracted from

  • Lin, F.P.; Ma, H.Y.; Lin, H.J.; Liu, S.M.; Tzou, W.S.
    Biochemical characterization of two truncated forms of amylopullulanase from Thermoanaerobacterium saccharolyticum NTOU1 to identify its enzymatically active region (2011), Appl. Biochem. Biotechnol., 165, 1047-1056.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) pLysS cells Thermoanaerobacterium saccharolyticum

Organism

Organism UniProt Comment Textmining
Thermoanaerobacterium saccharolyticum C5IGQ7
-
-
Thermoanaerobacterium saccharolyticum NTOU1 C5IGQ7
-
-

Purification (Commentary)

Purification (Comment) Organism
BD TALON spin column chromatography, gel filtration Thermoanaerobacterium saccharolyticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Thermoanaerobacterium saccharolyticum maltotriose + maltose + ?
-
?
amylopectin + H2O
-
Thermoanaerobacterium saccharolyticum NTOU1 maltotriose + maltose + ?
-
?
amylose + H2O
-
Thermoanaerobacterium saccharolyticum maltotriose + maltose
-
?
amylose + H2O
-
Thermoanaerobacterium saccharolyticum NTOU1 maltotriose + maltose
-
?
glycogen + H2O
-
Thermoanaerobacterium saccharolyticum maltotriose + maltose
-
?
additional information the enzyme cleaves both alpha-1,4- and alpha-1,6-glycosidic linkages in starch, pullulan, amylopectin, and other related oligosaccharides Thermoanaerobacterium saccharolyticum ?
-
?
additional information the enzyme cleaves both alpha-1,4- and alpha-1,6-glycosidic linkages in starch, pullulan, amylopectin, and other related oligosaccharides Thermoanaerobacterium saccharolyticum NTOU1 ?
-
?
pullulan + H2O
-
Thermoanaerobacterium saccharolyticum maltotriose + ?
-
?
pullulan + H2O
-
Thermoanaerobacterium saccharolyticum NTOU1 maltotriose + ?
-
?
soluble starch + H2O
-
Thermoanaerobacterium saccharolyticum maltotriose + maltose
-
?
soluble starch + H2O
-
Thermoanaerobacterium saccharolyticum NTOU1 maltotriose + maltose
-
?

Synonyms

Synonyms Comment Organism
amylopullulanase
-
Thermoanaerobacterium saccharolyticum
Apu
-
Thermoanaerobacterium saccharolyticum
ApuK885 truncated enzyme form Thermoanaerobacterium saccharolyticum
ApuM957 truncated enzyme form Thermoanaerobacterium saccharolyticum
pullulanase type II
-
Thermoanaerobacterium saccharolyticum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Thermoanaerobacterium saccharolyticum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
574.7
-
soluble starch truncated enzyme form ApuK855, at pH 6.0 and 70°C Thermoanaerobacterium saccharolyticum
877.9
-
soluble starch truncated enzyme form ApuM957, at pH 6.0 and 70°C Thermoanaerobacterium saccharolyticum
1751
-
pullulan truncated enzyme form ApuM957, at pH 5.0 and 70°C Thermoanaerobacterium saccharolyticum
1828
-
pullulan truncated enzyme form ApuK855, at pH 5.0 and 70°C Thermoanaerobacterium saccharolyticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
optimum pH for pullulanase activity Thermoanaerobacterium saccharolyticum
6
-
optimum pH for amylase activity Thermoanaerobacterium saccharolyticum