Cloned (Comment) | Organism |
---|---|
expression of His-tagged NPDE in Escherichia coli strain MC1061 | Nostoc punctiforme |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, mixing of 0.0012 ml of 14 mg/ml protein in 50 mM Na-phosphate buffer, pH 7.5, with 0.0012 ml reservoir solution containing 20% w/v PEG 8000, 200 mM Tris-HCl buffer, pH 8.5, and 100 mM MgCl2, X-ray diffraction structure determination and analysis at 2.37 A resolution, molecular structure modelling | Nostoc punctiforme |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Nostoc punctiforme | NPDE exhibits a unique catalytic preference for longer malto-oligosaccharides with more than 8 monomers, performing hydrolysis without the transgylcosylation or CD-hydrolyzing activities of other GH-13 enzymes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc punctiforme | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged NPDE from Escherichia coli strain MC1061 by nickel affinity chromatography to homogeneity and over 96% purity | Nostoc punctiforme |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NPDE exhibits a unique catalytic preference for longer malto-oligosaccharides with more than 8 monomers, performing hydrolysis without the transgylcosylation or CD-hydrolyzing activities of other GH-13 enzymes | Nostoc punctiforme | ? | - |
? | |
additional information | NPDE hydrolyzes both alpha-1,4- and alpha-1,6-glucosidic linkages with a substrate specificity in descending order of pullulan, amylopectin, starch, amylose. NPDE has no specificity for alpha-, beta-, and gamma-cyclodextris, and hydrolyzes alpha-1,6-glucosidic linkages more rapidly than alpha-1,4-glucosidic linkages. NPDE hydrolyzes the alpha-1,6 glycosidic linkages of malto-oligosaccharides, molecular basis for the substrate specificity and the catalytic properties, overview | Nostoc punctiforme | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | active form | Nostoc punctiforme |
monomer | inactive form | Nostoc punctiforme |
Synonyms | Comment | Organism |
---|---|---|
debranching enzyme | - |
Nostoc punctiforme |
More | the enzyme belongs to the glycosyl hydrolase 13 family, GH13 | Nostoc punctiforme |
Nostoc punctiforme debranching enzyme | - |
Nostoc punctiforme |
NPDE | - |
Nostoc punctiforme |