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Literature summary for 3.2.1.41 extracted from

  • Dumbrepatil, A.B.; Choi, J.H.; Park, J.T.; Kim, M.J.; Kim, T.J.; Woo, E.J.; Park, K.H.
    Structural features of the Nostoc punctiforme debranching enzyme reveal the basis of its mechanism and substrate specificity (2010), Proteins, 78, 348-356.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged NPDE in Escherichia coli strain MC1061 Nostoc punctiforme

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, mixing of 0.0012 ml of 14 mg/ml protein in 50 mM Na-phosphate buffer, pH 7.5, with 0.0012 ml reservoir solution containing 20% w/v PEG 8000, 200 mM Tris-HCl buffer, pH 8.5, and 100 mM MgCl2, X-ray diffraction structure determination and analysis at 2.37 A resolution, molecular structure modelling Nostoc punctiforme

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Nostoc punctiforme NPDE exhibits a unique catalytic preference for longer malto-oligosaccharides with more than 8 monomers, performing hydrolysis without the transgylcosylation or CD-hydrolyzing activities of other GH-13 enzymes ?
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?

Organism

Organism UniProt Comment Textmining
Nostoc punctiforme
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Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged NPDE from Escherichia coli strain MC1061 by nickel affinity chromatography to homogeneity and over 96% purity Nostoc punctiforme

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information NPDE exhibits a unique catalytic preference for longer malto-oligosaccharides with more than 8 monomers, performing hydrolysis without the transgylcosylation or CD-hydrolyzing activities of other GH-13 enzymes Nostoc punctiforme ?
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?
additional information NPDE hydrolyzes both alpha-1,4- and alpha-1,6-glucosidic linkages with a substrate specificity in descending order of pullulan, amylopectin, starch, amylose. NPDE has no specificity for alpha-, beta-, and gamma-cyclodextris, and hydrolyzes alpha-1,6-glucosidic linkages more rapidly than alpha-1,4-glucosidic linkages. NPDE hydrolyzes the alpha-1,6 glycosidic linkages of malto-oligosaccharides, molecular basis for the substrate specificity and the catalytic properties, overview Nostoc punctiforme ?
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?

Subunits

Subunits Comment Organism
dimer active form Nostoc punctiforme
monomer inactive form Nostoc punctiforme

Synonyms

Synonyms Comment Organism
debranching enzyme
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Nostoc punctiforme
More the enzyme belongs to the glycosyl hydrolase 13 family, GH13 Nostoc punctiforme
Nostoc punctiforme debranching enzyme
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Nostoc punctiforme
NPDE
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Nostoc punctiforme