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Literature summary for 3.2.1.41 extracted from

  • Malle,D.; Itoh, T.; Hashimoto, W.; Murata, K.; Utsumi, S.; Mikami, B.
    Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168 (2006), Acta Crystallogr. Sect. F, 62, 381-384.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.41

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging dop method, crystals belong to the orthorhombic space group P2(1)2(1)2(1) with unit-cell parameters a = 70.568, b = 127.68, c = 189.25 A. The crystal contains two molecules of pullulanase in the asymmetric unit, with a solvent content of 53.15% Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-value for pullulan is 1.284 mg/ml Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81000
-
 x * 81000, SDS-PAGE Bacillus subtilis
81100
-
 x * 81100, calculated from sequence Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis 168
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24.1
-
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pullulan + H2O
-
 Bacillus subtilis maltotriose + ?
-
 ?
pullulan + H2O
-
 Bacillus subtilis 168 maltotriose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 81000, SDS-PAGE Bacillus subtilis
? x * 81100, calculated from sequence Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
79
-
 pullulan pH 5.4 Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus subtilis