Cloned (Comment) | Organism |
---|---|
- |
Thermoanaerobacter ethanolicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-cyclodextrin | - |
Thermoanaerobacter ethanolicus | |
EDTA | - |
Thermoanaerobacter ethanolicus | |
N-bromosuccinimide | - |
Thermoanaerobacter ethanolicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation and stabilization | Thermoanaerobacter ethanolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
109000 | - |
x * 109000, SDS-PAGE | Thermoanaerobacter ethanolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter ethanolicus | - |
39E, bifunctional enzyme with activities of both alpha-amylase and pullulanase | - |
Purification (Comment) | Organism |
---|---|
recombinant protein expressed in Escherichia coli | Thermoanaerobacter ethanolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Thermoanaerobacter ethanolicus | malto-2-4-oligosaccharide + ? | - |
? | |
amylose + H2O | - |
Thermoanaerobacter ethanolicus | malto-2-4-oligosaccharide + ? | - |
? | |
glycogen + H2O | - |
Thermoanaerobacter ethanolicus | malto-2-4-oligosaccharide + ? | - |
? | |
pullulan + H2O | best substrate | Thermoanaerobacter ethanolicus | malto-2-4-oligosaccharide + ? | - |
? | |
soluble starch + H2O | - |
Thermoanaerobacter ethanolicus | malto-2-4-oligosaccharide + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 109000, SDS-PAGE | Thermoanaerobacter ethanolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
pullulanase activity | Thermoanaerobacter ethanolicus |
90 | - |
alpha-amylase activity | Thermoanaerobacter ethanolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
pullulanase activity | Thermoanaerobacter ethanolicus |
80 | - |
alpha-amylase activity | Thermoanaerobacter ethanolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermoanaerobacter ethanolicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | - |
Thermoanaerobacter ethanolicus |