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Literature summary for 3.2.1.41 extracted from
- Role of lipid modification on a starch-debranching enzyme, Klebsiella pullulanase: comparison of properties of lipid-modified and unmodified pullulanases (1992), Mol. Microbiol., 6, 389-394.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Klebsiella pneumoniae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Klebsiella aerogenes |  |
| Cu2+ | - |
Klebsiella pneumoniae | |
| Hg2+ | - |
Klebsiella aerogenes | |
| Hg2+ | - |
Klebsiella pneumoniae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
SDS-PAGE | Klebsiella aerogenes |
| 120000 | - |
SDS-PAGE | Klebsiella pneumoniae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Klebsiella aerogenes | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | Klebsiella pneumoniae | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | Klebsiella aerogenes | - |
maltotriose + ? | - |
? | |
| pullulan + H2O | Klebsiella pneumoniae | - |
maltotriose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
- |
- |
| Klebsiella pneumoniae | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| lipoprotein | - |
Klebsiella pneumoniae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the recombinant protein | Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Klebsiella aerogenes | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | - |
Klebsiella pneumoniae | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Klebsiella aerogenes | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Klebsiella pneumoniae | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | - |
Klebsiella aerogenes | maltotriose + ? | - |
? | |
| pullulan + H2O | - |
Klebsiella pneumoniae | maltotriose + ? | - |
? | |
| soluble starch | - |
Klebsiella aerogenes | maltotriose + maltose | - |
? | |
| soluble starch | - |
Klebsiella pneumoniae | maltotriose + maltose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pullulanase type II | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides | Klebsiella aerogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Klebsiella aerogenes |
| 60 | - |
- |
Klebsiella pneumoniae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Klebsiella aerogenes |
| 50 | 60 | - |
Klebsiella pneumoniae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Klebsiella aerogenes |
| 55 | - |
- |
Klebsiella pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Klebsiella aerogenes |
| 6 | - |
- |
Klebsiella pneumoniae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7.5 | - |
Klebsiella aerogenes |
| 4 | 7.5 | - |
Klebsiella pneumoniae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | - |
Klebsiella aerogenes |
| 3 | 11 | - |
Klebsiella pneumoniae |
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Release 2023.1 (January 2023)
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