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Literature summary for 3.2.1.41 extracted from
- Purification and general biochemical properties of thermostable pullulanase from Bacillus stearothermophilus G-82 (1992), Appl. Biochem. Biotechnol., 33, 193-203.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Geobacillus stearothermophilus |  |
| Ca2+ | - |
Geobacillus stearothermophilus | |
| Co2+ | - |
Geobacillus stearothermophilus | |
| Cu2+ | - |
Geobacillus stearothermophilus | |
| Fe3+ | - |
Geobacillus stearothermophilus | |
| Mg2+ | - |
Geobacillus stearothermophilus | |
| Mn2+ | - |
Geobacillus stearothermophilus | |
| N-bromosuccinimide | - |
Geobacillus stearothermophilus | |
| phenylmethylsulfonyl fluoride | partially | Geobacillus stearothermophilus | |
| Zn2+ | - |
Geobacillus stearothermophilus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Geobacillus stearothermophilus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | 56000 | gel filtration, gel electrophoresis | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Geobacillus stearothermophilus | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | Geobacillus stearothermophilus | - |
maltotriose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Geobacillus stearothermophilus | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Geobacillus stearothermophilus | maltooligosaccharides + maltose | - |
? | |
| amylose + H2O | weakly | Geobacillus stearothermophilus | maltooligosaccharides + maltose | - |
? | |
| dextrin + H2O | - |
Geobacillus stearothermophilus | maltooligosaccharides + maltose | - |
? | |
| glycogen + H2O | - |
Geobacillus stearothermophilus | maltooligosaccharides + maltose | - |
? | |
| maltoheptaose + H2O | - |
Geobacillus stearothermophilus | maltotriose + maltose + D-glucose | - |
? | |
| maltohexaose + H2O | - |
Geobacillus stearothermophilus | maltotriose + maltose + D-glucose + maltotetraose | - |
? | |
| maltopentaose + H2O | - |
Geobacillus stearothermophilus | maltotriose + maltose | - |
? | |
| maltotetraose + H2O | - |
Geobacillus stearothermophilus | maltose + glucose + maltotriose | - |
? | |
| pullulan + H2O | - |
Geobacillus stearothermophilus | maltotriose + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pullulanase type II | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 65 | - |
Geobacillus stearothermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 75 | - |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
no loss of activity after 10 min | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9 | - |
Geobacillus stearothermophilus |
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