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Literature summary for 3.2.1.41 extracted from
- Purification and characterization of a thermostable pullulanase from Thermoactinomyces thalpophilus (1988), J. Ind. Microbiol., 3, 343-350.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Laceyella sacchari |  |
| Ag+ | - |
Laceyella sacchari | |
| Ba2+ | - |
Laceyella sacchari | |
| Bi3+ | - |
Laceyella sacchari | |
| Cu2+ | - |
Laceyella sacchari | |
| EDTA | - |
Laceyella sacchari | |
| Fe2+ | - |
Laceyella sacchari | |
| Hg2+ | - |
Laceyella sacchari | |
| KMnO4 | - |
Laceyella sacchari | |
| Pb2+ | - |
Laceyella sacchari | |
| Sodium dodecyl sulfate | - |
Laceyella sacchari | |
| Sr2+ | - |
Laceyella sacchari | |
| Zn2+ | - |
Laceyella sacchari | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Laceyella sacchari | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | enhances stability | Laceyella sacchari | |
| Co2+ | - |
Laceyella sacchari | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 79000 | - |
gel filtration | Laceyella sacchari |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Laceyella sacchari | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | Laceyella sacchari | - |
maltotriose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Laceyella sacchari | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Laceyella sacchari |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1352 | 1790 | - |
Laceyella sacchari |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Laceyella sacchari | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Laceyella sacchari | maltooligosaccharides + maltose | - |
? | |
| amylopectin beta-limit dextrin + H2O | - |
Laceyella sacchari | maltotriose + maltose | - |
? | |
| glycogen beta-limit dextrin + H2O | - |
Laceyella sacchari | maltotriose + maltose + maltosetetraose | - |
? | |
| pullulan + H2O | - |
Laceyella sacchari | maltotriose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 79000, gel electrophoresis | Laceyella sacchari |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pullulanase type II | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides | Laceyella sacchari |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Laceyella sacchari |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Laceyella sacchari |
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