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Literature summary for 3.2.1.40 extracted from

  • De Lise, F.; Mensitieri, F.; Tarallo, V.; Ventimiglia, N.; Vinciguerra, R.; Tramice, A.; Marchetti, R.; Pizzo, E.; Notomista, E.; Cafaro, V.; Molinaro, A.; Birolo, L.; Di Donato, A.; Izzo, V.
    RHA-P Isolation, expression and characterization of a bacterial alpha-L-rhamnosidase from Novosphingobium sp. PP1Y (2016), J. Mol. Catal. B, 134, 136-147 .
No PubMed abstract available
Search for more literature for 3.2.1.40

Application

Application Comment Organism
biotechnology potential use as a biocatalyst for diverse biotechnological applications Novosphingobium sp. PP1Y

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Novosphingobium sp. PP1Y

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
160.2
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 6.9, temperature not specified in the publication Novosphingobium sp. PP1Y

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
101000
-
 gel filtration Novosphingobium sp. PP1Y

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 10% acetone, 50 mM MOPS, pH 6.9, 6.73% residual activity. Complete loss of activity in 50% acetone Novosphingobium sp. PP1Y
DMSO 10% DMSO, in 50 mM MOPS, pH 6.9, 66.29% residual activity. Complete loss of activity in 50% DMSO Novosphingobium sp. PP1Y
Ethanol 10% ethanol, 50 mM MOPS, pH 6.9, 66.52% residual activity. Complete loss of activity in 50% ethanol Novosphingobium sp. PP1Y

Organism

Organism UniProt Comment Textmining
Novosphingobium sp. PP1Y F6IEX3
-
-

Purification (Commentary)

Purification (Comment) Organism
expression in Escherichia coli, strain BL21(DE3) Novosphingobium sp. PP1Y

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Novosphingobium sp. PP1Y 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
naringin + H2O
-
 Novosphingobium sp. PP1Y beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
 ?
neohesperidin dihydrochalcone + H2O
-
 Novosphingobium sp. PP1Y 3,5-dihydroxy-4-[3-(3-hydroxy-4-methoxyphenyl)propanoyl]phenyl beta-D-glucopyranoside + alpha-L-rhamnose
-
 ?
rutin + H2O
-
 Novosphingobium sp. PP1Y isoquercitrin + alpha-L-rhamnose
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Novosphingobium sp. PP1Y

Synonyms

Synonyms Comment Organism
alpha-RHA
-
 Novosphingobium sp. PP1Y

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40.9
-
-
 Novosphingobium sp. PP1Y

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
32 45 32°C: about 55% of maximal activity, 45°C: about 65% of maximal activity Novosphingobium sp. PP1Y

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 40 3 h, stable Novosphingobium sp. PP1Y
40
-
 24 h, stable Novosphingobium sp. PP1Y

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.5
-
 Novosphingobium sp. PP1Y

pH Range

pH Minimum pH Maximum Comment Organism
7.9 8.8 the enzyme retains 47% and 36% of activity at pH 7.9 and 8.8 Novosphingobium sp. PP1Y