Literature summary for 3.2.1.40 extracted from

Li, L.; Gong, J.; Wang, S.; Li, G.; Gao, T.; Jiang, Z.; Cheng, Y.S.; Ni, H.; Li, Q.
Heterologous expression and characterization of a new clade of Aspergillus alpha-L-rhamnosidase suitable for citrus juice processing (2019), J. Agric. Food Chem., 67, 2926-2935 .

Search for more literature for 3.2.1.40

Application

Application	Comment	Organism
food industry	the enzyme can efficiently remove naringin from pomelo juice without changing its aroma. It is desirable for debittering citrus juice thereby improving the quality of juice	Aspergillus tubingensis

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris GS115	Aspergillus tubingensis

Inhibitors

Inhibitors	Comment	Organism	Structure
Hg2+	10 mM	Aspergillus tubingensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.47	-	naringin	pH 4.0, 60°C	Aspergillus tubingensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
110000	-	gel filtration	Aspergillus tubingensis

Organism

Organism	UniProt	Comment	Textmining
Aspergillus tubingensis	A0A1B2K4A7	-	-
Aspergillus tubingensis JMU-TS529	A0A1B2K4A7	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	the enzyme is secreted in the glycosylated form, which contains approximately 14 kDa of glycosidic chain	Aspergillus tubingensis

Purification (Commentary)

Purification (Comment)	Organism
-	Aspergillus tubingensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
naringin + H2O	the enzyme shows a strong ability to hydrolyze naringin but scarcely acts on other substrates. It shows negligible activities on rutin, hesperidin, quercitrin, ginsenoside Rg2, myricitrin, saikosaponin C and 4-nitrophenyl-alpha-L-rhamnoside	Aspergillus tubingensis	beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose	-	?
naringin + H2O	the enzyme shows a strong ability to hydrolyze naringin but scarcely acts on other substrates. It shows negligible activities on rutin, hesperidin, quercitrin, ginsenoside Rg2, myricitrin, saikosaponin C and 4-nitrophenyl-alpha-L-rhamnoside	Aspergillus tubingensis JMU-TS529	beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 110000, SDS-PAGE	Aspergillus tubingensis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	60	-	Aspergillus tubingensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
48900	-	naringin	pH 4.0, 60°C	Aspergillus tubingensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	-	-	Aspergillus tubingensis

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Release 2023.1 (January 2023)