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Literature summary for 3.2.1.40 extracted from

  • Ge, L.; Xie, J.; Wu, T.; Zhang, S.; Zhao, L.; Ding, G.; Wang, Z.; Xiao, W.
    Purification and characterisation of a novel alpha-L-rhamnosidase exhibiting transglycosylating activity from Aspergillus oryzae (2017), Int. J. Food Sci. Technol., 52, 2596-2603 .
No PubMed abstract available
Search for more literature for 3.2.1.40

Application

Application Comment Organism
food industry the enzyme exhibits transglycosylating activity, which can synthesise rhamnosyl mannitol through the reactions of transglycosylation with inexpensive rhamnose as the glycosyl donor. The enzyme has potential value for glycoside synthesis in the food industry Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 10 mM, 17% loss of activity Aspergillus oryzae
Cu2+ 10 mM, 68% loss of activity Aspergillus oryzae
Fe2+ 10 mM, 54% loss of activity Aspergillus oryzae
Fe3+ 10 mM, 97% loss of activity Aspergillus oryzae
Hg2+ 10 mM, 99% loss of activity Aspergillus oryzae
K+ 10 mM, 5% loss of activity Aspergillus oryzae
Li+ 10 mM, 8% loss of activity Aspergillus oryzae
Mn2+ 10 mM, 5% loss of activity Aspergillus oryzae
Na+ 10 mM, 10% loss of activity Aspergillus oryzae
Ni2+ 10 mM, 41% loss of activity Aspergillus oryzae
Zn2+ 10 mM, 13 % loss of activity Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.2
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 4.5, 65°C Aspergillus oryzae

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 10 mM, 1.1fold activation Aspergillus oryzae
Ca2+ 10 mM, activation to 105% of control Aspergillus oryzae
Mg2+ 10 mM, activation to 104% of control Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 SDS-PAGE Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-
Aspergillus oryzae NL-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
224.4
-
 pH 4.5, 65°C Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Aspergillus oryzae 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Aspergillus oryzae NL-1 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
hesperidin + H2O 240.6% of the activity with rutin Aspergillus oryzae hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
 ?
hesperidin + H2O 240.6% of the activity with rutin Aspergillus oryzae NL-1 hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
 ?
additional information no activity with myricetrin Aspergillus oryzae ?
-
 ?
additional information no activity with myricetrin Aspergillus oryzae NL-1 ?
-
 ?
naringin + H2O 21% of the activity with rutin Aspergillus oryzae beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
 ?
naringin + H2O 21% of the activity with rutin Aspergillus oryzae NL-1 beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
 ?
rutin + H2O
-
 Aspergillus oryzae isoquercitrin + alpha-L-rhamnose
-
 ?
rutin + H2O
-
 Aspergillus oryzae NL-1 isoquercitrin + alpha-L-rhamnose
-
 ?

Subunits

Subunits Comment Organism
? x * 75000, SDS-PAGE Aspergillus oryzae

Synonyms

Synonyms Comment Organism
AoRha
-
 Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Aspergillus oryzae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 75 activity is more than 50% of the maximum activity in the temperature range 45°C-75°C Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 24 h, residual activity is more than 60% Aspergillus oryzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1624
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 4.5, 65°C Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
3.5 5.5 at pH ranging from 3.5 to 5.5 still the enzyme maintains more than 60% of the optimal pH activity Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 7 stable in the pH range 3.5-7.0 Aspergillus oryzae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
312
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 4.5, 65°C Aspergillus oryzae