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Literature summary for 3.2.1.40 extracted from

  • Guillotin, L.; Kim, H.; Traore, Y.; Moreau, P.; Lafite, P.; Coquoin, V.; Nuccio, S.; De Vaumas, R.; Daniellou, R.
    Biochemical characterization of the alpha-L-rhamnosidase DtRha from Dictyoglomus thermophilum application to the selective derhamnosylation of natural flavonoids (2019), ACS Omega, 4, 1916-1922 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
-
Dictyoglomus thermophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
4-nitrophenyl alpha-L-rhamnopyranoside pH 5.0, 37°C Dictyoglomus thermophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
106000
-
calculated from sequence Dictyoglomus thermophilum

Organism

Organism UniProt Comment Textmining
Dictyoglomus thermophilum B5YC64
-
-
Dictyoglomus thermophilum ATCC 35947 B5YC64
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Dictyoglomus thermophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
Dictyoglomus thermophilum 4-nitrophenol + alpha-L-rhamnopyranose
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
Dictyoglomus thermophilum ATCC 35947 4-nitrophenol + alpha-L-rhamnopyranose
-
?

Synonyms

Synonyms Comment Organism
DtRha
-
Dictyoglomus thermophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
Dictyoglomus thermophilum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 90 hydrolysis of 4-nitrophenyl-alpha-L-rhamnopyranoside at 37°C is catalyzed at 17% of the activity compared to that at 90°C Dictyoglomus thermophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.17
-
4-nitrophenyl alpha-L-rhamnopyranoside pH 5.0, 37°C Dictyoglomus thermophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Dictyoglomus thermophilum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1
-
4-nitrophenyl alpha-L-rhamnopyranoside pH 5.0, 37°C Dictyoglomus thermophilum