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Literature summary for 3.2.1.40 extracted from

  • Birgisson, H.; Hreggvidsson, G.O.; Fridjonsson, O.H.; Mort, A.; Kristjansson, J.K.; Mattiasson, B.
    Two new thermostable alpha-L-rhamnosidases from a novel thermophilic bacterium (2004), Enzyme Microb. Technol., 34, 561-571.
No PubMed abstract available

Application

Application Comment Organism
nutrition alpha-L-rhamnosidases are used in wine production Thermomicrobia bacterium

Cloned(Commentary)

Cloned (Comment) Organism
gene rhmA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Thermomicrobia bacterium
gene rhmB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Thermomicrobia bacterium

Inhibitors

Inhibitors Comment Organism Structure
D-glucose about 500 mM D-glucose concentration in grape juice inhibits RhmA by 39%; about 500 mM D-glucose concentration in grape juice inhibits RhmB by 38% Thermomicrobia bacterium
ethanol at 12% v/v, 51% inhibition of RhmB; at 12% v/v, 60% inhibition of RhmA Thermomicrobia bacterium
L-rhamnose complete inhibition, competitive product inhibition; complete inhibition, competitive product inhibition Thermomicrobia bacterium
Zn2+ 30-35% inhibition at 2 mM, reversible by 10 mM EDTA Thermomicrobia bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Thermomicrobia bacterium
0.46
-
4-nitrophenyl alpha-L-rhamnopyranoside pH 7.5, 50°C, recombinant RhmA Thermomicrobia bacterium
0.66
-
4-nitrophenyl alpha-L-rhamnopyranoside pH 7.0, 50°C, recombinant RhmB Thermomicrobia bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no effect by EDTA, Mg2+, Ca2+, and Mn2+ on RhmB Thermomicrobia bacterium
additional information no effect by EDTA, Zn2+, Mg2+, Ca2+, and Mn2+ on RhmA Thermomicrobia bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
104000
-
2 * 104000, recombinant enzyme, SDS-PAGE Thermomicrobia bacterium
107000
-
2 * 107000, recombinant enzyme, SDS-PAGE Thermomicrobia bacterium
210000
-
recombinant enzyme, gel filtration Thermomicrobia bacterium

Organism

Organism UniProt Comment Textmining
Thermomicrobia bacterium Q6RCI8 gene rhmB, isozyme RhmB
-
Thermomicrobia bacterium Q6RCI9 gene rhmA, isozyme RhmA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 63fold from Escherichia coli by heat treatment at 60°C, hydrophobic interaction and ion exchange chromatography Thermomicrobia bacterium
recombinant enzyme 85fold from Escherichia coli by heat treatment at 60°C, hydrophobic interaction and ion exchange chromatography Thermomicrobia bacterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
108.9
-
purified recombinant enzyme Thermomicrobia bacterium
263.9
-
purified recombinant enzyme Thermomicrobia bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
Thermomicrobia bacterium 4-nitrophenol + alpha-L-rhamnose
-
?
hesperidin + H2O cleavage of the alpha-1,6-linkage Thermomicrobia bacterium ? + alpha-L-rhamnose
-
?
additional information no activity with quercitrin Thermomicrobia bacterium ?
-
?
additional information no activity with rutin and quercitrin Thermomicrobia bacterium ?
-
?
naringin + H2O preferred substrate, cleavage of the alpha-1,2-linkage Thermomicrobia bacterium ? + alpha-L-rhamnose
-
?
rhamnogalacturonan tetramer + H2O
-
Thermomicrobia bacterium ?
-
?
rutin + H2O low activity, cleavage of the alpha-1,6-linkage Thermomicrobia bacterium ? + alpha-L-rhamnose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 104000, recombinant enzyme, SDS-PAGE Thermomicrobia bacterium
dimer 2 * 107000, recombinant enzyme, SDS-PAGE Thermomicrobia bacterium

Synonyms

Synonyms Comment Organism
alpha-L-rhamnosidase A
-
Thermomicrobia bacterium
alpha-L-rhamnosidase B
-
Thermomicrobia bacterium
RhmA
-
Thermomicrobia bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Thermomicrobia bacterium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 low activity above Thermomicrobia bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
completely stable for 24 h at pH 5.0-6.9 Thermomicrobia bacterium
40
-
loss of 22% activity within 24 h at pH 7.9 Thermomicrobia bacterium
60
-
20-30% remaining activity after 24 h at pH 5.0-6.9 Thermomicrobia bacterium
60
-
20-30% remaining activity after 24 h at pH 7.9 Thermomicrobia bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6.9
-
Thermomicrobia bacterium
7.9
-
-
Thermomicrobia bacterium

pH Range

pH Minimum pH Maximum Comment Organism
4 7.9 50% of maximal activity at pH 4.0 and pH 7.9 Thermomicrobia bacterium
5 8.7 50% of maximal activity at pH 5.0 and pH 8.7 Thermomicrobia bacterium

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.28
-
L-rhamnose pH 7.5, 50°C, recombinant RhmA, versus 4-nitrophenyl alpha-L-rhamnopyranoside Thermomicrobia bacterium
4.65
-
L-rhamnose pH 7.0, 50°C, recombinant RhmB, versus 4-nitrophenyl alpha-L-rhamnopyranoside Thermomicrobia bacterium

pI Value

Organism Comment pI Value Maximum pI Value
Thermomicrobia bacterium sequence calculation
-
4.5
Thermomicrobia bacterium sequence calculation
-
4.6