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Literature summary for 3.2.1.40 extracted from

  • Hashimoto, W.; Nankai, H.; Sato, N.; Kawai, S.; Murata, K.
    Characterization of alpha-L-rhamnosidase of Bacillus sp. GL1 responsible for the complete depolymerization of gellan (1999), Arch. Biochem. Biophys., 368, 56-60.
    View publication on PubMed
Search for more literature for 3.2.1.40

General Stability

General Stability Organism
pepstatin stabilizes Bacillus sp. (in: Bacteria)
phenylmethanesulfonyl fluorid stabilizes Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 native gradient PAGE, SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-L-rhamnoside + H2O Bacillus sp. (in: Bacteria) alpha-L-rhamnose in glycolipids or glycosides alpha-L-rhamnose + ?
-
 ?
alpha-L-rhamnoside + H2O Bacillus sp. (in: Bacteria) GL1 alpha-L-rhamnose in glycolipids or glycosides alpha-L-rhamnose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 GL1
-
Bacillus sp. (in: Bacteria) GL1
-
 GL1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Bacillus sp. (in: Bacteria)
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-L-rhamnoside + H2O alpha-L-rhamnose in glycolipids or glycosides Bacillus sp. (in: Bacteria) alpha-L-rhamnose + ?
-
 ?
alpha-L-rhamnoside + H2O alpha-L-rhamnose in glycolipids or glycosides Bacillus sp. (in: Bacteria) GL1 alpha-L-rhamnose + ?
-
 ?
p-nitrophenyl alpha-L-rhamnoside + H2O
-
 Bacillus sp. (in: Bacteria) p-nitrophenol + alpha-L-rhamnose
-
 ?
p-nitrophenyl alpha-L-rhamnoside + H2O
-
 Bacillus sp. (in: Bacteria) GL1 p-nitrophenol + alpha-L-rhamnose
-
 ?
rhamnosyl-glucose + H2O gellan as carbon-source Bacillus sp. (in: Bacteria) glucose + alpha-L-rhamnose
-
 ?
rhamnosyl-glucose + H2O gellan as carbon-source Bacillus sp. (in: Bacteria) GL1 glucose + alpha-L-rhamnose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 100000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 80% loss of activity after 10 min, pH 7 Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bacillus sp. (in: Bacteria)