Literature summary for 3.2.1.4 extracted from

Schroeder, C.; Burkhardt, C.; Busch, P.; Schirrmacher, G.; Claren, J.; Antranikian, G.
Characterization of a theme C glycoside hydrolase family 9 endo-beta-glucanase from a biogas reactor metagenome (2018), Protein J., 37, 454-460 .

Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
gene bp_cel9A, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21	uncultured bacterium

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	5 or 10 mM of EDTA reduce barley beta-glucan hydrolysis by 39% and 34%, respectively	uncultured bacterium
EGTA	10 mM, 24% inhibition	uncultured bacterium
Zn2+	73% inhibition at 10 mM	uncultured bacterium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates 40% at 5 mM and 31% at 10 mM	uncultured bacterium
Mg2+	activates 31% at 5 mM	uncultured bacterium
additional information	the impact of metal ions Ca2+ and Mg2+ is more significant at higher temperatures	uncultured bacterium

Organism

Organism	UniProt	Comment	Textmining
uncultured bacterium	A0A346PYQ5	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
24	-	purified recombinant His-tagged enzyme, substrate barley beta-glucan, pH 6.0, 30°C	uncultured bacterium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme is active with beta-D-glucans containing (1->3)- and (1->4)-bonds (EC 3.2.1.73, licheninase) and with beta-D-glucans containing only (1->4)-bonds (EC 3.2.1.4, cellulase). It shows an exclusive endoacting mechanism. No activity with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-cellobioside, avicel, curdlan, laminarin, cellulose, carboxymethyl cellulose, and xylan	uncultured bacterium	?	-	?
xyloglucan + H2O	a beta-1,4-1,6-glucan, 40% activity compared to barley beta-glucan	uncultured bacterium	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 62600, about, recombinant enzyme, sequence calculation	uncultured bacterium
More	domain organization, lack of a carbohydrate binding module	uncultured bacterium

Synonyms

Synonyms	Comment	Organism
BP_Cel9A	-	uncultured bacterium
endo-beta-glucanase	-	uncultured bacterium
glycoside hydrolase family 9 endoglucanase	-	uncultured bacterium
More	cf. EC 3.2.1.73	uncultured bacterium
theme C glycoside hydrolase family 9 endo-beta-glucanase	-	uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	uncultured bacterium

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
5	40	the enzyme shows 88% and 77% of maximal activity at 20°C and 40°C, and 63% and 38% of maximal activity at 10°C and at 5°C	uncultured bacterium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	assay at	uncultured bacterium

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8	maximal activity at pH 6.0, 95% activity at pH 7.0, and 66% activity at pH 8.0	uncultured bacterium

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the theme C glycoside hydrolase family 9, GH9. Theme C glucanases exhibit an N-terminal Ig domain	uncultured bacterium
additional information	the putative catalytic residues are Asp147, Asp150 and Glu517	uncultured bacterium

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)