Literature summary for 3.2.1.4 extracted from

Li, L.; Huang, C.; Zhao, F.; Deng, T.; Lin, Y.; Zheng, S.; Liang, S.; Han, S.
Improved production and characterization of Volvariella volvacea endoglucanase 1 expressed in Pichia pastoris (2018), Protein Expr. Purif., 152, 107-113 .

Activating Compound

Activating Compound	Comment	Organism	Structure
DTT	24.1% activation at 10 mM	Volvariella volvacea

Cloned(Commentary)

Cloned (Comment)	Organism
gene eg1, recombinant expression in Pichia pastoris strains GS115 and X33, enzyme EG1 folding and secretion efficiency from the endoplasmic reticulum is best enhanced by co-overexpression of chaperone HAC1 in the host strain GS115 harboring eight EG1 copies, subcloning in Escherichia coli strain TOP10, quantitative PCR expression analysis, method evaluation and optimization for high enzyme production	Volvariella volvacea

Cloned (Comment)

Organism

gene eg1, recombinant expression in Pichia pastoris strains GS115 and X33, enzyme EG1 folding and secretion efficiency from the endoplasmic reticulum is best enhanced by co-overexpression of chaperone HAC1 in the host strain GS115 harboring eight EG1 copies, subcloning in Escherichia coli strain TOP10, quantitative PCR expression analysis, method evaluation and optimization for high enzyme production

Volvariella volvacea

Inhibitors

Inhibitors	Comment	Organism
Ca2+	12.7% inhibition at 10 mM	Volvariella volvacea
Co2+	10.1% inhibition at 10 mM	Volvariella volvacea
Cu2+	37.7% inhibition at 10 mM	Volvariella volvacea
Ni2+	12.1% inhibition at 10 mM	Volvariella volvacea
SDS	52.1% inhibition at 1%	Volvariella volvacea

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	KM is 14.3 mg/ml, substrate carboxymethyl cellulose, recombinant enzyme, pH 5.0, 60°C	Volvariella volvacea

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	the enzyme is secreted	Volvariella volvacea	-	-

Metals/Ions

Metals/Ions	Comment	Organism
Ba2+	26.1% activation at 10 mM	Volvariella volvacea
K+	32.8% activation at 10 mM	Volvariella volvacea
Li+	5.2% activation at 10 mM	Volvariella volvacea
Mg2+	5.1% activation at 10 mM	Volvariella volvacea
Mn2+	25.8% activation at 10 mM	Volvariella volvacea
additional information	no effect by Al3+ at 10 mM	Volvariella volvacea
Zn2+	10.0% activation at 10 mM	Volvariella volvacea

Organism

Organism	UniProt	Comment	Textmining
Volvariella volvacea	B3GQ73	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
carboxymethyl cellulose + H2O	-	Volvariella volvacea	?	-	?

Synonyms

Synonyms	Comment	Organism
EG1	-	Volvariella volvacea
endoglucanase 1	-	Volvariella volvacea

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	recombinant enzyme	Volvariella volvacea

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
74.51	-	carboxymethyl cellulose	recombinant enzyme, pH 5.0, 60°C	Volvariella volvacea

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	recombinant enzyme	Volvariella volvacea

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kcat/KM is 312.9 ml*mg/min, substrate carboxymethyl cellulose, recombinant enzyme, pH 5.0, 60°C	Volvariella volvacea