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Literature summary for 3.2.1.4 extracted from

  • Bashirova, A.; Pramanik, S.; Volkov, P.; Rozhkova, A.; Nemashkalov, V.; Zorov, I.; Gusakov, A.; Sinitsyn, A.; Schwaneberg, U.; Davari, M.D.
    Disulfide bond engineering of an endoglucanase from Penicillium verruculosum to improve its thermostability (2019), Int. J. Mol. Sci., 20, E1602 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information molecular dynamics simulations reveal that the introduced disulfide bond rigidified a global structure of DSB2 and DSB3 mutant variants, thus enhancing their thermostability Talaromyces verruculosus
S127C/A165C site-directed mutagenesis, introduction of a disulfide bond into mutant DSB2, the mutant enzyme displays 15-21% increased specific activity against carboxymethylcellulose and beta-glucan, and increased thermostability compared to wild-type enzyme EGLII Talaromyces verruculosus
Y171C/L201C site-directed mutagenesis, introduction of a disulfide bond into mutant DSB3, the mutant enzyme displays 15-21% increased specific activity against carboxymethylcellulose and beta-glucan, and increased thermostability compared to wild-type enzyme EGLII Talaromyces verruculosus

Organism

Organism UniProt Comment Textmining
Talaromyces verruculosus A0A1U7Q1U3 i.e. Penicillium verruculosum
-
Talaromyces verruculosus ATCC 10513 A0A1U7Q1U3 i.e. Penicillium verruculosum
-
Talaromyces verruculosus CBS 388.48 A0A1U7Q1U3 i.e. Penicillium verruculosum
-
Talaromyces verruculosus DSM 2263 A0A1U7Q1U3 i.e. Penicillium verruculosum
-
Talaromyces verruculosus IMI 040039 A0A1U7Q1U3 i.e. Penicillium verruculosum
-
Talaromyces verruculosus NRRL 1050 A0A1U7Q1U3 i.e. Penicillium verruculosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
53
-
purified recombinant wild-type enzyme, substrate beta-glucan, pH and temperature not specified in the publication Talaromyces verruculosus
57
-
purified recombinant wild-type enzyme, substrate carboxymethyl cellulose, pH and temperature not specified in the publication Talaromyces verruculosus
61
-
purified recombinant mutant S127C/A165C, substrate beta-glucan, pH and temperature not specified in the publication Talaromyces verruculosus
64
-
purified recombinant mutant Y171C/L201C, substrate beta-glucan, pH and temperature not specified in the publication Talaromyces verruculosus
66
-
purified recombinant mutant Y171C/L201C, substrate carboxymethyl cellulose, pH and temperature not specified in the publication Talaromyces verruculosus
68
-
purified recombinant mutant S127C/A165C, substrate carboxymethyl cellulose, pH and temperature not specified in the publication Talaromyces verruculosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-glucan + H2O
-
Talaromyces verruculosus ?
-
?
beta-glucan + H2O
-
Talaromyces verruculosus DSM 2263 ?
-
?
beta-glucan + H2O
-
Talaromyces verruculosus NRRL 1050 ?
-
?
beta-glucan + H2O
-
Talaromyces verruculosus CBS 388.48 ?
-
?
beta-glucan + H2O
-
Talaromyces verruculosus ATCC 10513 ?
-
?
beta-glucan + H2O
-
Talaromyces verruculosus IMI 040039 ?
-
?
carboxymethyl cellulose + H2O
-
Talaromyces verruculosus ?
-
?
carboxymethyl cellulose + H2O
-
Talaromyces verruculosus DSM 2263 ?
-
?
carboxymethyl cellulose + H2O
-
Talaromyces verruculosus NRRL 1050 ?
-
?
carboxymethyl cellulose + H2O
-
Talaromyces verruculosus CBS 388.48 ?
-
?
carboxymethyl cellulose + H2O
-
Talaromyces verruculosus ATCC 10513 ?
-
?
carboxymethyl cellulose + H2O
-
Talaromyces verruculosus IMI 040039 ?
-
?

Synonyms

Synonyms Comment Organism
EGLII
-
Talaromyces verruculosus
endoglucanase
-
Talaromyces verruculosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
2 h, the wild-type enzyme retains 38% activity, the enzyme mutants retain 52% and 58% activity, respectively Talaromyces verruculosus
80
-
2 h, the wild-type enzyme retains no activity, the enzyme mutants retain 15% and 22% activity, respectively Talaromyces verruculosus

General Information

General Information Comment Organism
additional information catalytic residues are E142 and E249 in the enzyme active site. The enzyme contains three potential disulfide bonds Talaromyces verruculosus