Protein Variants | Comment | Organism |
---|---|---|
additional information | molecular dynamics simulations reveal that the introduced disulfide bond rigidified a global structure of DSB2 and DSB3 mutant variants, thus enhancing their thermostability | Talaromyces verruculosus |
S127C/A165C | site-directed mutagenesis, introduction of a disulfide bond into mutant DSB2, the mutant enzyme displays 15-21% increased specific activity against carboxymethylcellulose and beta-glucan, and increased thermostability compared to wild-type enzyme EGLII | Talaromyces verruculosus |
Y171C/L201C | site-directed mutagenesis, introduction of a disulfide bond into mutant DSB3, the mutant enzyme displays 15-21% increased specific activity against carboxymethylcellulose and beta-glucan, and increased thermostability compared to wild-type enzyme EGLII | Talaromyces verruculosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Talaromyces verruculosus | A0A1U7Q1U3 | i.e. Penicillium verruculosum | - |
Talaromyces verruculosus ATCC 10513 | A0A1U7Q1U3 | i.e. Penicillium verruculosum | - |
Talaromyces verruculosus CBS 388.48 | A0A1U7Q1U3 | i.e. Penicillium verruculosum | - |
Talaromyces verruculosus DSM 2263 | A0A1U7Q1U3 | i.e. Penicillium verruculosum | - |
Talaromyces verruculosus IMI 040039 | A0A1U7Q1U3 | i.e. Penicillium verruculosum | - |
Talaromyces verruculosus NRRL 1050 | A0A1U7Q1U3 | i.e. Penicillium verruculosum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
53 | - |
purified recombinant wild-type enzyme, substrate beta-glucan, pH and temperature not specified in the publication | Talaromyces verruculosus |
57 | - |
purified recombinant wild-type enzyme, substrate carboxymethyl cellulose, pH and temperature not specified in the publication | Talaromyces verruculosus |
61 | - |
purified recombinant mutant S127C/A165C, substrate beta-glucan, pH and temperature not specified in the publication | Talaromyces verruculosus |
64 | - |
purified recombinant mutant Y171C/L201C, substrate beta-glucan, pH and temperature not specified in the publication | Talaromyces verruculosus |
66 | - |
purified recombinant mutant Y171C/L201C, substrate carboxymethyl cellulose, pH and temperature not specified in the publication | Talaromyces verruculosus |
68 | - |
purified recombinant mutant S127C/A165C, substrate carboxymethyl cellulose, pH and temperature not specified in the publication | Talaromyces verruculosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-glucan + H2O | - |
Talaromyces verruculosus | ? | - |
? | |
beta-glucan + H2O | - |
Talaromyces verruculosus DSM 2263 | ? | - |
? | |
beta-glucan + H2O | - |
Talaromyces verruculosus NRRL 1050 | ? | - |
? | |
beta-glucan + H2O | - |
Talaromyces verruculosus CBS 388.48 | ? | - |
? | |
beta-glucan + H2O | - |
Talaromyces verruculosus ATCC 10513 | ? | - |
? | |
beta-glucan + H2O | - |
Talaromyces verruculosus IMI 040039 | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Talaromyces verruculosus | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Talaromyces verruculosus DSM 2263 | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Talaromyces verruculosus NRRL 1050 | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Talaromyces verruculosus CBS 388.48 | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Talaromyces verruculosus ATCC 10513 | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Talaromyces verruculosus IMI 040039 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EGLII | - |
Talaromyces verruculosus |
endoglucanase | - |
Talaromyces verruculosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
2 h, the wild-type enzyme retains 38% activity, the enzyme mutants retain 52% and 58% activity, respectively | Talaromyces verruculosus |
80 | - |
2 h, the wild-type enzyme retains no activity, the enzyme mutants retain 15% and 22% activity, respectively | Talaromyces verruculosus |
General Information | Comment | Organism |
---|---|---|
additional information | catalytic residues are E142 and E249 in the enzyme active site. The enzyme contains three potential disulfide bonds | Talaromyces verruculosus |