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Literature summary for 3.2.1.4 extracted from

  • Basit, A.; Liu, J.; Miao, T.; Zheng, F.; Rahim, K.; Lou, H.; Jiang, W.
    Characterization of two endo-beta-1, 4-xylanases from Myceliophthora thermophila and their saccharification efficiencies, synergistic with commercial cellulase (2018), Front. Microbiol., 9, 233 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Co2+ 20.6% activation at 1 mM Pseudoalteromonas sp. DY3
Mn2+ 28.8% activation at 1 mM Pseudoalteromonas sp. DY3

Cloned(Commentary)

Cloned (Comment) Organism
gene celX, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudoalteromonas sp. DY3

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 6.5% inhibition at 1 mM Pseudoalteromonas sp. DY3
Pb2+ 14.9% inhibition at 1 mM Pseudoalteromonas sp. DY3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km for carboxymethyl cellulose is 6.4 mg/ml, pH 5.5, 45°C, recombinant enzyme, Michaelis-Menten kinetics Pseudoalteromonas sp. DY3

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no or poor effect by 1 mM of Mg2+, Ba2+, Zn2+, Ca2+, and Ni2+ Pseudoalteromonas sp. DY3

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas sp. DY3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Pseudoalteromonas sp. DY3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
Pseudoalteromonas sp. DY3 ?
-
?

Subunits

Subunits Comment Organism
? x * 52200, recombinant His-tagged enzyme, SDS-PAGE Pseudoalteromonas sp. DY3

Synonyms

Synonyms Comment Organism
CelX
-
Pseudoalteromonas sp. DY3
endoglucanase
-
Pseudoalteromonas sp. DY3

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
recombinant enzyme Pseudoalteromonas sp. DY3

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 45 34.7% of maximal activity at 5°C, maximal activity at 45°C, rapid loss of activity above. The cold adaptation of enzyme CelX is possibly due to the presence of the unusually long linker between the catalytic module and the cellulose-binding domain Pseudoalteromonas sp. DY3

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 55 purified recombinant enzyme, very stable at 25°C, loss of about 13.2% activity at 35°C after 2 h. The enzyme retains 10.4% activity at 45°C after 2 h, and is completely inactivated at 55°C after 30 min Pseudoalteromonas sp. DY3

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2
-
carboxymethyl cellulose pH 5.5, 45°C, recombinant enzyme Pseudoalteromonas sp. DY3

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
recombinant enzyme Pseudoalteromonas sp. DY3

pH Range

pH Minimum pH Maximum Comment Organism
5 9 over 76.9% of maximal activity within this range, no activity at pH 4.0 and below, 59.2% and 42.6% of maximal activity at pH 10.0 and pH 11.0 respectively Pseudoalteromonas sp. DY3

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
purified recombinant His-tagged enzyme, inactivation after 1 h Pseudoalteromonas sp. DY3
5 11 purified recombinant His-tagged enzyme, 120 h, 83.0% activity remaining, 66% at pH 11.0 after 120 h Pseudoalteromonas sp. DY3