Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 20.6% activation at 1 mM | Pseudoalteromonas sp. DY3 | |
Mn2+ | 28.8% activation at 1 mM | Pseudoalteromonas sp. DY3 |
Cloned (Comment) | Organism |
---|---|
gene celX, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Pseudoalteromonas sp. DY3 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 6.5% inhibition at 1 mM | Pseudoalteromonas sp. DY3 | |
Pb2+ | 14.9% inhibition at 1 mM | Pseudoalteromonas sp. DY3 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for carboxymethyl cellulose is 6.4 mg/ml, pH 5.5, 45°C, recombinant enzyme, Michaelis-Menten kinetics | Pseudoalteromonas sp. DY3 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no or poor effect by 1 mM of Mg2+, Ba2+, Zn2+, Ca2+, and Ni2+ | Pseudoalteromonas sp. DY3 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas sp. DY3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Pseudoalteromonas sp. DY3 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + H2O | - |
Pseudoalteromonas sp. DY3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52200, recombinant His-tagged enzyme, SDS-PAGE | Pseudoalteromonas sp. DY3 |
Synonyms | Comment | Organism |
---|---|---|
CelX | - |
Pseudoalteromonas sp. DY3 |
endoglucanase | - |
Pseudoalteromonas sp. DY3 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant enzyme | Pseudoalteromonas sp. DY3 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 45 | 34.7% of maximal activity at 5°C, maximal activity at 45°C, rapid loss of activity above. The cold adaptation of enzyme CelX is possibly due to the presence of the unusually long linker between the catalytic module and the cellulose-binding domain | Pseudoalteromonas sp. DY3 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | purified recombinant enzyme, very stable at 25°C, loss of about 13.2% activity at 35°C after 2 h. The enzyme retains 10.4% activity at 45°C after 2 h, and is completely inactivated at 55°C after 30 min | Pseudoalteromonas sp. DY3 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
carboxymethyl cellulose | pH 5.5, 45°C, recombinant enzyme | Pseudoalteromonas sp. DY3 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
recombinant enzyme | Pseudoalteromonas sp. DY3 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | over 76.9% of maximal activity within this range, no activity at pH 4.0 and below, 59.2% and 42.6% of maximal activity at pH 10.0 and pH 11.0 respectively | Pseudoalteromonas sp. DY3 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
purified recombinant His-tagged enzyme, inactivation after 1 h | Pseudoalteromonas sp. DY3 |
5 | 11 | purified recombinant His-tagged enzyme, 120 h, 83.0% activity remaining, 66% at pH 11.0 after 120 h | Pseudoalteromonas sp. DY3 |