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Literature summary for 3.2.1.4 extracted from

  • Silva, T.P.; de Albuquerque, F.S.; Dos Santos, C.W.V.; Franco, M.; Caetano, L.C.; Pereira, H.J.V.
    Production, purification, characterization and application of a new halotolerant and thermostable endoglucanase of Botrytis ricini URM 5627 (2018), Biores. Technol., 270, 263-269 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Amphobotrys ricini
Cu2+
-
Amphobotrys ricini
Fe2+
-
Amphobotrys ricini

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information the enzyme has a Km of 0.1299 mg/ml and a Vmax of 0.097 mol/min/ml, Michaelis-Menten kinetic modeling Amphobotrys ricini

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Amphobotrys ricini
Mn2+ activates Amphobotrys ricini
Na+ activates Amphobotrys ricini
NaCl the endoglucanase revealed a halotolerant profile since its activity increased proportionally to an increase in NaCl concentration. The maximum activity is reached at 2 M NaCl with a 75% increase in activity Amphobotrys ricini
Zn2+ activates Amphobotrys ricini

Organism

Organism UniProt Comment Textmining
Amphobotrys ricini
-
-
-
Amphobotrys ricini URM 5627
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by ammonium sulfate fractionation and gel filtration Amphobotrys ricini

Source Tissue

Source Tissue Comment Organism Textmining
mycelium solid fermentation of the fungus Botrytis ricini strain URM 5627 on a resin made of sugarcane bagasse, coconut fibre, and wood powder Amphobotrys ricini
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
purified native enzyme, 1289.83 U/ml, pH 5.0, 50°C Amphobotrys ricini
57.92
-
purified native enzyme, pH 5.0, 50°C Amphobotrys ricini

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O 2% solution Amphobotrys ricini ?
-
?
carboxymethyl cellulose + H2O 2% solution Amphobotrys ricini URM 5627 ?
-
?
additional information the enzyme is able to hydrolyse sugarcane bagasse, rice husk, and wheat bran, with the highest production of reducers/fermentable sugars within 24 h of saccharification for wheat bran (137.21 mg/g). Saccharification of agroindustrial residues, overview Amphobotrys ricini ?
-
?
additional information the enzyme is able to hydrolyse sugarcane bagasse, rice husk, and wheat bran, with the highest production of reducers/fermentable sugars within 24 h of saccharification for wheat bran (137.21 mg/g). Saccharification of agroindustrial residues, overview Amphobotrys ricini URM 5627 ?
-
?

Subunits

Subunits Comment Organism
? x * 39000, SDS-PAGE Amphobotrys ricini

Synonyms

Synonyms Comment Organism
CMCase
-
Amphobotrys ricini
endo-1,4-beta-D-glucanase
-
Amphobotrys ricini
endoglucanase
-
Amphobotrys ricini

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Amphobotrys ricini

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
39 60 the purified enzyme is stable at 39-60°C and pH 4.0-6.0 for 60 min Amphobotrys ricini

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Amphobotrys ricini

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6 the purified enzyme is stable at 39-60°C and pH 4.0-6.0 for 60 min Amphobotrys ricini

General Information

General Information Comment Organism
additional information halotolerant endoglucanase Amphobotrys ricini