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Literature summary for 3.2.1.4 extracted from
- Biochemical characterization and low-resolution SAXS structure of two-domain endoglucanase BlCel9 from Bacillus licheniformis (2019), Appl. Microbiol. Biotechnol., 103, 1275-1287 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cel9 or celA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bacillus licheniformis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| enzyme structure analysis using small-angle X-ray scattering at 30 A resolution and homology modelling with the crystallographic model of the endoglucanase TfCel9A (PDB ID 1JS4), structure comparisons | Bacillus licheniformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight inhibition | Bacillus licheniformis |  |
| Co2+ | moderate inhibition | Bacillus licheniformis | |
| Cu2+ | strong inhibition | Bacillus licheniformis | |
| DMSO | moderate inhibition | Bacillus licheniformis | |
| EDTA | slight inhibition | Bacillus licheniformis | |
| Fe2+ | strong inhibition | Bacillus licheniformis | |
| K+ | slight inhibition | Bacillus licheniformis | |
| Li+ | slight inhibition | Bacillus licheniformis | |
| Mn2+ | slight inhibition | Bacillus licheniformis | |
| Ni2+ | slight inhibition | Bacillus licheniformis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | Q65JI9 | - |
- |
| Bacillus licheniformis ATCC 14580 | Q65JI9 | - |
- |
| Bacillus licheniformis DSM 13 | Q65JI9 | - |
- |
| Bacillus licheniformis Gibson 46 | Q65JI9 | - |
- |
| Bacillus licheniformis JCM 2505 | Q65JI9 | - |
- |
| Bacillus licheniformis NBRC 12200 | Q65JI9 | - |
- |
| Bacillus licheniformis NCIMB 9375 | Q65JI9 | - |
- |
| Bacillus licheniformis NRRL NRS-1264 | Q65JI9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by TEV protease, again nickel affinity chromatography, and gel filtration | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bacterial cellulose + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| bacterial cellulose + H2O | - |
Bacillus licheniformis NCIMB 9375 | ? | - |
? | |
| bacterial cellulose + H2O | - |
Bacillus licheniformis Gibson 46 | ? | - |
? | |
| bacterial cellulose + H2O | - |
Bacillus licheniformis JCM 2505 | ? | - |
? | |
| bacterial cellulose + H2O | - |
Bacillus licheniformis NRRL NRS-1264 | ? | - |
? | |
| bacterial cellulose + H2O | - |
Bacillus licheniformis NBRC 12200 | ? | - |
? | |
| bacterial cellulose + H2O | - |
Bacillus licheniformis ATCC 14580 | ? | - |
? | |
| bacterial cellulose + H2O | - |
Bacillus licheniformis DSM 13 | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Bacillus licheniformis NCIMB 9375 | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Bacillus licheniformis Gibson 46 | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Bacillus licheniformis JCM 2505 | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Bacillus licheniformis NRRL NRS-1264 | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Bacillus licheniformis NBRC 12200 | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Bacillus licheniformis ATCC 14580 | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Bacillus licheniformis DSM 13 | ? | - |
? | |
| filter paper + H2O | qualitative filter paper-grade 1 | Bacillus licheniformis | ? | - |
? | |
| filter paper + H2O | qualitative filter paper-grade 1 | Bacillus licheniformis NCIMB 9375 | ? | - |
? | |
| filter paper + H2O | qualitative filter paper-grade 1 | Bacillus licheniformis Gibson 46 | ? | - |
? | |
| filter paper + H2O | qualitative filter paper-grade 1 | Bacillus licheniformis JCM 2505 | ? | - |
? | |
| filter paper + H2O | qualitative filter paper-grade 1 | Bacillus licheniformis NRRL NRS-1264 | ? | - |
? | |
| filter paper + H2O | qualitative filter paper-grade 1 | Bacillus licheniformis NBRC 12200 | ? | - |
? | |
| filter paper + H2O | qualitative filter paper-grade 1 | Bacillus licheniformis ATCC 14580 | ? | - |
? | |
| filter paper + H2O | qualitative filter paper-grade 1 | Bacillus licheniformis DSM 13 | ? | - |
? | |
| additional information | substrate specificity, overview. Poor activity with Avicel PH-101 | Bacillus licheniformis | ? | - |
? | |
| additional information | substrate specificity, overview. Poor activity with Avicel PH-101 | Bacillus licheniformis NCIMB 9375 | ? | - |
? | |
| additional information | substrate specificity, overview. Poor activity with Avicel PH-101 | Bacillus licheniformis Gibson 46 | ? | - |
? | |
| additional information | substrate specificity, overview. Poor activity with Avicel PH-101 | Bacillus licheniformis JCM 2505 | ? | - |
? | |
| additional information | substrate specificity, overview. Poor activity with Avicel PH-101 | Bacillus licheniformis NRRL NRS-1264 | ? | - |
? | |
| additional information | substrate specificity, overview. Poor activity with Avicel PH-101 | Bacillus licheniformis NBRC 12200 | ? | - |
? | |
| additional information | substrate specificity, overview. Poor activity with Avicel PH-101 | Bacillus licheniformis ATCC 14580 | ? | - |
? | |
| additional information | substrate specificity, overview. Poor activity with Avicel PH-101 | Bacillus licheniformis DSM 13 | ? | - |
? | |
| phosphoric acid swollen cellulose + H2O | best substrate | Bacillus licheniformis | D-glucose + cellobiose + cellotriose + cellotetraose | - |
? | |
| phosphoric acid swollen cellulose + H2O | best substrate | Bacillus licheniformis NCIMB 9375 | D-glucose + cellobiose + cellotriose + cellotetraose | - |
? | |
| phosphoric acid swollen cellulose + H2O | best substrate | Bacillus licheniformis Gibson 46 | D-glucose + cellobiose + cellotriose + cellotetraose | - |
? | |
| phosphoric acid swollen cellulose + H2O | best substrate | Bacillus licheniformis JCM 2505 | D-glucose + cellobiose + cellotriose + cellotetraose | - |
? | |
| phosphoric acid swollen cellulose + H2O | best substrate | Bacillus licheniformis NRRL NRS-1264 | D-glucose + cellobiose + cellotriose + cellotetraose | - |
? | |
| phosphoric acid swollen cellulose + H2O | best substrate | Bacillus licheniformis NBRC 12200 | D-glucose + cellobiose + cellotriose + cellotetraose | - |
? | |
| phosphoric acid swollen cellulose + H2O | best substrate | Bacillus licheniformis ATCC 14580 | D-glucose + cellobiose + cellotriose + cellotetraose | - |
? | |
| phosphoric acid swollen cellulose + H2O | best substrate | Bacillus licheniformis DSM 13 | D-glucose + cellobiose + cellotriose + cellotetraose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BlCel9 | - |
Bacillus licheniformis |
| endoglucanase | - |
Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bacillus licheniformis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 75 | over 50% of maximal activity within this range | Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified recombinant enzyme, completely stable to slightly activated after 48 h | Bacillus licheniformis |
| 50 | - |
purified recombinant enzyme, 80% activity remaining after 48 h | Bacillus licheniformis |
| 60 | - |
purified recombinant enzyme, 50% activity remaining after 3 h, 20% after 6 h, and inactivation after 12 h | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bacillus licheniformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8.2 | over 50% of maximal activity within this range | Bacillus licheniformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
purified recombinant enzyme, Tm is 35°C | Bacillus licheniformis |
| 5 | 9 | purified recombinant enzyme, Tm is about 75°C within this range | Bacillus licheniformis |
| 10 | - |
purified recombinant enzyme, Tm is 55°C | Bacillus licheniformis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | enzyme structure analysis using small-angle X-ray scattering and homology modelling with the crystallographic model of the endoglucanase TfCel9A (PDB ID 1JS4), structure comparisons | Bacillus licheniformis |
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Release 2023.1 (January 2023)
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