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Literature summary for 3.2.1.4 extracted from
- Extremely thermoactive archaeal endoglucanase from a shallow marine hydrothermal vent from Vulcano Island (2019), Appl. Microbiol. Biotechnol., 103, 1267-1274 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 24.7% activation at 5 mM | uncultured archaeon |  |
| additional information | no or poor effect by 5 mM of SDS, DTT, and EDTA | uncultured archaeon | |
| Tween 20 | 9.3% activation at 5 mM | uncultured archaeon | |
| Tween 80 | 11.7% activation at 5 mM | uncultured archaeon | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene vul_cel5A, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain C43 | uncultured archaeon |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inhibition at 5 mM | uncultured archaeon | |
| Ca2+ | 23.8% inhibition at 5 mM | uncultured archaeon | |
| Cu2+ | complete inhibition at 5 mM | uncultured archaeon | |
| Fe2+ | complete inhibition at 5 mM | uncultured archaeon | |
| Fe3+ | 61.4% inhibition at 5 mM | uncultured archaeon | |
| K+ | 10.2% inhibition at 5 mM | uncultured archaeon | |
| Mg2+ | 8.1% inhibition at 5 mM | uncultured archaeon | |
| Mn2+ | 59.3% inhibition at 5 mM | uncultured archaeon | |
| additional information | no or poor effect by 5 mM of SDS, DTT, and EDTA | uncultured archaeon | |
| NaCl | 7.1% inhibition at 5 mM | uncultured archaeon | |
| Urea | 9.7% inhibition at 5 mM | uncultured archaeon | |
| Zn2+ | complete inhibition at 5 mM | uncultured archaeon | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme contains a signal peptide and is secreted | uncultured archaeon | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured archaeon | A0A3Q9CZQ1 | from a shallow marine hydrothermal vent from Vulcano Island | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain C43 by nickel affinity chromatography | uncultured archaeon |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 38.5 | - |
purified recombinant His-tagged enzyme, pH 6.0, 115°C, substrate carboxymethyl cellulose | uncultured archaeon |
| 209.9 | - |
purified recombinant His-tagged enzyme, pH 6.0, 115°C, substrate lichenan | uncultured archaeon |
| 210.7 | - |
purified recombinant His-tagged enzyme, pH 6.0, 115°C, substrate barley beta-glucan | uncultured archaeon |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-glucan + H2O | best substrate, high activity, product analysis | uncultured archaeon | cellobiose + cellotriose + D-glucose | - |
? | |
| carboxymethyl cellulose + H2O | soluble, lower activity | uncultured archaeon | ? | - |
? | |
| lichenan + H2O | high activity | uncultured archaeon | ? | - |
? | |
| locust bean gum + H2O | - |
uncultured archaeon | ? | - |
? | |
| additional information | no activity towards the insoluble substrates crystalline cellulose and Avicel, and against curdlan, laminarin, xyloglucan, and xylan | uncultured archaeon | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55400, about, sequence calculation | uncultured archaeon |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cel5A | - |
uncultured archaeon |
| endo-beta-1,4-glucanase | - |
uncultured archaeon |
| endoglucanase | - |
uncultured archaeon |
| Vul_Cel5A | - |
uncultured archaeon |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 115 | - |
- |
uncultured archaeon |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 130 | activity range, profile overview | uncultured archaeon |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
purified recombinant His-tagged enzyme, pH 6.0, completely stable for 48 h | uncultured archaeon |
| 100 | - |
purified recombinant His-tagged enzyme, pH 6.0, half-life is 46 min | uncultured archaeon |
| 115 | - |
purified recombinant His-tagged enzyme, pH 6.0, half-life is 9.1 min | uncultured archaeon |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
uncultured archaeon |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | activity range, profile overview | uncultured archaeon |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | purified recombinant His-tagged enzyme, 75°C, 95-100% activity remaining after 24 h at pH 5.0-7.0, 41% at pH 3.0 and 45% at pH 4.0 after 24 h, complete loss of activity at pH 2.5 and pH 9 | uncultured archaeon |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| uncultured archaeon | sequence calculation | - |
4.95 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycoside hydrolase family 5, GH5. The GH5 signature pattern PS00659 (174 IGADIKNEPH 185) is identified within the sequence of Vul_Cel5A | uncultured archaeon |
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