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Literature summary for 3.2.1.4 extracted from
- Functional characterization of a thermostable endoglucanase belonging to glycoside hydrolase family 45 from Fomitopsis palustris (2018), Appl. Microbiol. Biotechnol., 102, 6515-6523 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Fpcel45a, DNA and amino acid sequence determination and analysis, phylogenetic tree of fungal proteins belonging to GH family 45, recombinant expression of His-tagged enzyme in Pichia pastoris strain GS115 | Fomitopsis palustris |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D117N | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Fomitopsis palustris |
| N95D | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Fomitopsis palustris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fomitopsis palustris | D2YZL5 | - |
- |
| Fomitopsis palustris FFPRI 0507 | D2YZL5 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | lack of glycosylation is determined using PNGase F | Fomitopsis palustris |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography | Fomitopsis palustris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | grown on potato dextrose agar | Fomitopsis palustris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 24 | - |
purified recombinant His-tagged enzyme, substrate lichenan, pH 5.0, 50°C | Fomitopsis palustris |
| 32 | - |
purified recombinant His-tagged enzyme, substrate beta-glucan, pH 5.0, 50°C | Fomitopsis palustris |
| 64 | - |
purified recombinant His-tagged enzyme, substrate glucomannan, pH 5.0, 50°C | Fomitopsis palustris |
| 79 | - |
purified recombinant His-tagged enzyme, substrate carboxymethyl cellulose, pH 5.0, 50°C | Fomitopsis palustris |
| 87 | - |
purified recombinant His-tagged enzyme, substrate phosphoric acid swollen cellulose, pH 5.0, 50°C | Fomitopsis palustris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-glucan + H2O | - |
Fomitopsis palustris | ? | - |
? |  |
| beta-glucan + H2O | - |
Fomitopsis palustris FFPRI 0507 | ? | - |
? | |
| carboxymethyl cellulose + H2O | high activity | Fomitopsis palustris | ? | - |
? | |
| carboxymethyl cellulose + H2O | high activity | Fomitopsis palustris FFPRI 0507 | ? | - |
? | |
| glucomannan + H2O | - |
Fomitopsis palustris | ? | - |
? | |
| glucomannan + H2O | - |
Fomitopsis palustris FFPRI 0507 | ? | - |
? | |
| lichenan + H2O | - |
Fomitopsis palustris | ? | - |
? | |
| lichenan + H2O | - |
Fomitopsis palustris FFPRI 0507 | ? | - |
? | |
| additional information | the enzyme has a unique catalytic efficiency on beta-1,4-glucans rather than mixed beta-1,3/1,4-glucans as compared to other GH45 endoglucanases. No activity on Avicel and 4-nitrophenyl beta-D-cellobioside | Fomitopsis palustris | ? | - |
? | |
| additional information | the enzyme has a unique catalytic efficiency on beta-1,4-glucans rather than mixed beta-1,3/1,4-glucans as compared to other GH45 endoglucanases. No activity on Avicel and 4-nitrophenyl beta-D-cellobioside | Fomitopsis palustris FFPRI 0507 | ? | - |
? | |
| phosphoric acid swollen cellulose + H2O | best substrate | Fomitopsis palustris | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, about, recombinant His-tagged enzyme, SDS-PAGE | Fomitopsis palustris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoglucanase | - |
Fomitopsis palustris |
| FpCel45 | - |
Fomitopsis palustris |
| Fpcel45a | - |
Fomitopsis palustris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Fomitopsis palustris |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 65 | over 50% activity within this range, profile overview | Fomitopsis palustris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | purified recombinant His-tagged enzyme, half-life of enzyme is 168 h at 60°C and 3 h at 70°C, respectively | Fomitopsis palustris |
| 80 | - |
purified recombinant His-tagged enzyme, at least 91.4% activity remains after 1 h | Fomitopsis palustris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Fomitopsis palustris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | over 50% activity within this range, profile overview | Fomitopsis palustris |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the subfamily C of glycoside hydrolase family 45, GH45 | Fomitopsis palustris |
| additional information | residues N95D and D117N are both essential for the catalytic reaction. Enzyme structure homology modelling using the crystal structure of endoglucanase V-like protein PcCel45A (PDB code 3X2N) as a template. Asn92 as catalytic residue is conserved in FpCel45 | Fomitopsis palustris |
| physiological function | endoglucanases randomly hydrolyze the internal beta-1,4-glycosidic linkages of cellulose into cellooligosaccharides to facilitate subsequent processes | Fomitopsis palustris |
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