Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.4 extracted from

  • Zeng, X.; Xue, D.
    Increasing of activity and thermostability of cold active butanol-tolerant endoglucanase from a marine Rhodococcus sp. under high concentrations of butanol condition (2018), 3 Biotech, 8, 265 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.4

Activating Compound

Activating Compound Comment Organism Structure
butanol butanol-tolerant endoglucanase, enzyme activity is 1.18fold increased in 5% butanol solution compared to butanol-free solution. Melting point temperature is 2.5°C higher in 5% butanol solution (50°C) than in butanol-free solution (47.5°C). At 45-60°C, the Gbbs-free energy change, DELTAG, is higher in 5% butanol solution than that in butanol-free solution Rhodococcus sp.
additional information no effect by DTT and EDTA at 10 mM Rhodococcus sp.

Application

Application Comment Organism
synthesis the cold active butanol-tolerant endoglucanase is valuable for biobutanol production by a simultaneous saccharification and fermentation process Rhodococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 14% inhibition at 10 mM Rhodococcus sp.
Cu2+ 22% inhibition at 10 mM Rhodococcus sp.
additional information no effect by DTT and EDTA at 10 mM Rhodococcus sp.
Pb2+ 65% inhibition at 10 mM Rhodococcus sp.
Zn2+ 74% inhibition at 10 mM Rhodococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information thermodynamic kinetics of the endoglucanase at different concentrations of butanol Rhodococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no or poor effect by Ca2+, Na+, K+, Mg2+, and Fe2+ Rhodococcus sp.

Organic Solvent Stability

Organic Solvent Comment Organism
Butanol butanol-tolerant endoglucanase, enzyme activity is 1.18fold increased in 5% butanol solution compared to butanol-free solution. Melting point temperature is 2.5°C higher in 5% butanol solution (50°C) than in butanol-free solution (47.5°C). At 45-60°C, the Gibbs-free energy change, DELTAG, is higher in 5% butanol solution than that in butanol-free solution. The content of alpha-helix and beta-sheet increases in 5% butanol solution Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O best substrate Rhodococcus sp. ?
-
 ?
cellulose + H2O filter paper, 18% activity compared to carboxymethyl cellulose Rhodococcus sp. ?
-
 ?
additional information no activity with Avicel, cotton, laminarin, and cellobiose Rhodococcus sp. ?
-
 ?

Subunits

Subunits Comment Organism
More the content of alpha-helix and beta-sheet increases in 5% butanol solution. The increasing of alpha-helix and beta-sheet content leads to higher activity and better thermostability in butanol solution Rhodococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Rhodococcus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 cold-active endoglucanase Rhodococcus sp.
10 70 and above, activity range, profile overview. Over 70% activity at 30-65°C Rhodococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Rhodococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
3 7 over 80% of maximal activity within this range Rhodococcus sp.