Literature summary for 3.2.1.4 extracted from

Gagoski, D.; Shi, Z.; Nielsen, L.K.; Vickers, C.E.; Mahler, S.; Speight, R.; Johnston, W.A.; Alexandrov, K.
Cell-free pipeline for discovery of thermotolerant xylanases and endo-1,4-beta-glucanases (2017), J. Biotechnol., 259, 191-198 .

Search for more literature for 3.2.1.4

Application

Application	Comment	Organism
analysis	construction of a pipeline based on Leishmania tarentolae cell-free system to characterize 30 putative thermostable endo-1,4-beta-glucanases and xylanases identified in public genomic databases. The system uses high-throughput assays for glucanase and xylanase activities that rely on solubilisation of labelled particulate substrates performed in multiwell plates	Bacillus subtilis
analysis	construction of a pipeline based on Leishmania tarentolae cell-free system to characterize 30 putative thermostable endo-1,4-beta-glucanases and xylanases identified in public genomic databases. The system uses high-throughput assays for glucanase and xylanase activities that rely on solubilisation of labelled particulate substrates performed in multiwell plates	Castellaniella defragrans
analysis	construction of a pipeline based on Leishmania tarentolae cell-free system to characterize 30 putative thermostable endo-1,4-beta-glucanases and xylanases identified in public genomic databases. The system uses high-throughput assays for glucanase and xylanase activities that rely on solubilisation of labelled particulate substrates performed in multiwell plates	Halosimplex carlsbadense

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bacillus subtilis
expression in Escherichia coli	Castellaniella defragrans
expression in Escherichia coli	Halosimplex carlsbadense

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	-	-
Castellaniella defragrans	-	-	-
Halosimplex carlsbadense	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
azurine-labelled hydroxyethylcellulose + H2O	-	Bacillus subtilis	?	-	?
azurine-labelled hydroxyethylcellulose + H2O	-	Castellaniella defragrans	?	-	?
azurine-labelled hydroxyethylcellulose + H2O	-	Halosimplex carlsbadense	?	-	?

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Release 2023.1 (January 2023)