Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Thermochaetoides thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
carboxymethylcellulose | Km value 0.060 mg/ml, pH 4.0, 70°C | Thermochaetoides thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermochaetoides thermophila | V9PD44 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | recombinant protein expressed in Pichia pastoris | Thermochaetoides thermophila |
Renatured (Comment) | Organism |
---|---|
recombinant protein | Thermochaetoides thermophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.52 | - |
substrate carboxymethylcellulose, pH 4.0, 70°C | Thermochaetoides thermophila |
2.14 | - |
substrate beta-1,4-D-glucan, pH 4.0, 70°C | Thermochaetoides thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-D-glucan + H2O | - |
Thermochaetoides thermophila | ? | - |
? | |
carboxymethylcellulose + H2O | - |
Thermochaetoides thermophila | ? | - |
? | |
pectin + H2O | - |
Thermochaetoides thermophila | galacturonic acid oligosaccharides | - |
? | |
soluble starch + H2O | - |
Thermochaetoides thermophila | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE, x * 24500, calculated | Thermochaetoides thermophila |
Synonyms | Comment | Organism |
---|---|---|
CBH45-1 | - |
Thermochaetoides thermophila |
CTendo45 | - |
Thermochaetoides thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermochaetoides thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 h, stabel | Thermochaetoides thermophila |
70 | - |
1 h, 79% residual activtiy | Thermochaetoides thermophila |
80 | - |
1 h, 66% residual activity | Thermochaetoides thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Thermochaetoides thermophila |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermochaetoides thermophila | calculated | - |
5.9 |