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Literature summary for 3.2.1.4 extracted from
- Improving the activity of the endoglucanase, Cel8M from Escherichia coli by error-prone PCR (2016), Enzyme Microb. Technol., 86, 52-58 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G117S | 1.6fold increase in activity, mutation might directly affect the substratebinding affinity | Escherichia coli |
| V9A/K353E | 1.4fold increase in activity, mutation might directly affect the substratebinding affinity | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
carboxymethylcellulose | Km value is 280.31 mg/ml, 222.74 mg/ml, and 110.22 mg/ml for wild-type and mutants V9A/K353E and G117S, respectively, pH 7.0, 40°C | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P37651 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | - |
Escherichia coli | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BcsZ | - |
Escherichia coli |
| Cel8M | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
1 h, 70% residual activity | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
carboxymethylcellulose | mutant V9A/K353E, pH 7.0, 40°C | Escherichia coli | |
| 0.16 | - |
carboxymethylcellulose | wild-type, pH 7.0, 40°C | Escherichia coli | |
| 0.17 | - |
carboxymethylcellulose | mutant G117S, pH 7.0, 40°C | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Escherichia coli |
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Release 2023.1 (January 2023)
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