Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.4 extracted from

  • Tong, C.C.; Cole, A.L.; Shepherd, M.G.
    Purification and properties of the cellulases from the thermophilic fungus Thermoascus aurantiacus (1980), Biochem. J., 191, 83-94.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
carboxymethylcellulose 1.9 mg/ml, cellulase III, pH 4.5, 70°C Thermoascus aurantiacus
additional information
-
carboxymethylcellulose 3.9 mg/ml, cellulase I, pH 4.5, 70°C Thermoascus aurantiacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
gel filtration, cellulase III Thermoascus aurantiacus
48000
-
gel filtration, cellulase II Thermoascus aurantiacus
51000
-
-
Thermoascus aurantiacus
78000
-
gel filtration, cellulase I Thermoascus aurantiacus

Organism

Organism UniProt Comment Textmining
Thermoascus aurantiacus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein cellulase I contains 5.5% carbohydrate. Cellulase II conatins 2.6% carbohydrate. Cellulase III contains 1.8% carbohydrate Thermoascus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O
-
Thermoascus aurantiacus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 34000, cellulase III Thermoascus aurantiacus
monomer 1 * 51000, cellulase II Thermoascus aurantiacus

Synonyms

Synonyms Comment Organism
cellulase II
-
Thermoascus aurantiacus
cellulases I
-
Thermoascus aurantiacus
cellulases III
-
Thermoascus aurantiacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
cellulase III, substrate: carboxymethylcellulose Thermoascus aurantiacus
75
-
cellulase I, substrate: carboxymethylcellulose Thermoascus aurantiacus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 80 55°C: about 45% of maximal activity, 80°C: about 80% of maximal activity, cellulase I, substrate: carboxymethylcellulose Thermoascus aurantiacus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
completely stable up to 65°C Thermoascus aurantiacus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 12 25°C, 24 h, cellulase II, stable Thermoascus aurantiacus
4 8 25°C, 24 h, cellulase I, stable Thermoascus aurantiacus
6 9 25°C, 24 h, cellulase III, stable Thermoascus aurantiacus