Literature summary for 3.2.1.4 extracted from

Okano, H.; Kanaya, E.; Ozaki, M.; Angkawidjaja, C.; Kanaya, S.
Structure, activity, and stability of metagenome-derived glycoside hydrolase family 9 endoglucanase with an N-terminal Ig-like domain (2015), Protein Sci., 24, 408-419.

Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	uncultured bacterium

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 2.15 A resolution. Residues Asp194 and Asp197 form hydrogen bonds with a water molecule that may function as a nucleophile	uncultured bacterium

Protein Variants

Protein Variants	Comment	Organism
D99A	106% of wild-type activity, temperature of the midpoint of the thermal denaturation transition is decreased by 1.9°C	uncultured bacterium
additional information	a mutant lacking the immunoglobulin-like domain shows 1% of wild-type activity and a decrease in the temperature of the midpoint of the thermal denaturation transition by 6.3°C	uncultured bacterium
Q40A	94% of wild-type activity	uncultured bacterium
Q40A/D99A	97% of wild-type activity, temperature of the midpoint of the thermal denaturation transition is decreased by 5.0°C	uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	gel filtration	uncultured bacterium
62600	-	1 * 62600, calculated	uncultured bacterium

Organism

Organism	UniProt	Comment	Textmining
uncultured bacterium	W8PF21	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
34.4	-	wild-type, pH 7.0, 50°C	uncultured bacterium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
carboxymethyl cellulose + H2O	-	uncultured bacterium	?	-	?
additional information	no substrate: 4-nitrophenyl cellobioside	uncultured bacterium	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 62600, calculated	uncultured bacterium

Synonyms

Synonyms	Comment	Organism
CelG	-	uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	-	uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
81.4	-	denaturation temperature	uncultured bacterium

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	9	more than 80% of maximum activity	uncultured bacterium

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Release 2023.1 (January 2023)