Literature summary for 3.2.1.4 extracted from

Bayram Akcapinar, G.; Venturini, A.; Martelli, P.L.; Casadio, R.; Sezerman, U.O.
Modulating the thermostability of endoglucanase I from Trichoderma reesei using computational approaches (2015), Protein Eng. Des. Sel., 28, 127-135.

Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Trichoderma reesei

Protein Variants

Protein Variants	Comment	Organism
K272F	mutation predicted to be thermostabilizing. Both high temperature and room temperature molecular dynamics simulations supported a stabilizing effect. Mutant exhibits higher thermostability compared with native EGI, but the specific activity of the mutant is lower	Trichoderma reesei
Q126F	mutation predicted to be thermostabilizing. Both high temperature and room temperature molecular dynamics simulations supported a stabilizing effect. Mutant exhibits higher thermostability compared with native EGI, but the specific activity of the mutant is lower	Trichoderma reesei
Q274V	mutation predicted to be thermostabilizing. Both high temperature and room temperature molecular dynamics simulations supported a stabilizing effect. Mutant exhibits higher thermostability compared with native EGI, but the specific activity of the mutant is lower	Trichoderma reesei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	4-methylumbelliferyl cellobioside	mutant Q274V, pH 5.0, 45°C	Trichoderma reesei
0.111	-	4-methylumbelliferyl cellobioside	mutant K272F, pH 5.0, 45°C	Trichoderma reesei
0.114	-	4-methylumbelliferyl cellobioside	mutant Q126F, pH 5.0, 45°C	Trichoderma reesei
0.125	-	4-methylumbelliferyl cellobioside	wild-type, pH 5.0, 45°C	Trichoderma reesei

Organism

Organism	UniProt	Comment	Textmining
Trichoderma reesei	P07981	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl cellobioside + H2O	-	Trichoderma reesei	4-methylumbelliferone + cellobiose	-	?

Synonyms

Synonyms	Comment	Organism
Egl1	-	Trichoderma reesei
endoglucanase EG-I	-	Trichoderma reesei

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.001	-	4-methylumbelliferyl cellobioside	mutant K272F, pH 5.0, 45°C	Trichoderma reesei
0.001	-	4-methylumbelliferyl cellobioside	mutant Q274V, pH 5.0, 45°C	Trichoderma reesei
0.003	-	4-methylumbelliferyl cellobioside	mutant Q126F, pH 5.0, 45°C	Trichoderma reesei
0.01	-	4-methylumbelliferyl cellobioside	wild-type, pH 5.0, 45°C	Trichoderma reesei

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.01	-	4-methylumbelliferyl cellobioside	mutant K272F, pH 5.0, 45°C	Trichoderma reesei
0.01	-	4-methylumbelliferyl cellobioside	mutant Q274V, pH 5.0, 45°C	Trichoderma reesei
0.02	-	4-methylumbelliferyl cellobioside	mutant Q126F, pH 5.0, 45°C	Trichoderma reesei
0.08	-	4-methylumbelliferyl cellobioside	wild-type, pH 5.0, 45°C	Trichoderma reesei

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Release 2023.1 (January 2023)