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Literature summary for 3.2.1.4 extracted from

  • Xu, X.; Li, J.; Zhang, W.; Huang, H.; Shi, P.; Luo, H.; Liu, B.; Zhang, Y., Zhang, Z., Fan, Y.; Yao, B.
    A neutral thermostable beta-1,4-glucanase from Humicola insolens Y1 with potential for applications in various industries (2015), PLoS One, 10, e0124925.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Humicola insolens

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 86% residual activity Humicola insolens
cetyltrimethylammonium bromide 1 mM, 31% residual activity Humicola insolens
EDTA 1 mM, 91% residual activity Humicola insolens
Mn2+ 1 mM, 59% residual activity Humicola insolens
sodium dodecylsulfate 1 mM, 76% residual activity Humicola insolens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 Barley beta-glucan Km-value 1.29 mg/ml, pH 6.5, 70°C Humicola insolens

Organism

Organism UniProt Comment Textmining
Humicola insolens A0A0F7Q1T8
-
-
Humicola insolens Y1 A0A0F7Q1T8
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
372
-
 substrate barley beta-glucan, pH 6.5, 70°C Humicola insolens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
 Humicola insolens ?
-
 ?
barley beta-glucan + H2O
-
 Humicola insolens Y1 ?
-
 ?
carboxymethyl cellulose + H2O 13.6% of the activity with barley beta-glucan Humicola insolens ?
-
 ?
carboxymethyl cellulose + H2O 13.6% of the activity with barley beta-glucan Humicola insolens Y1 ?
-
 ?
lichenan + H2O 60.5% of the activity with barley beta-glucan Humicola insolens ?
-
 ?
lichenan + H2O 60.5% of the activity with barley beta-glucan Humicola insolens Y1 ?
-
 ?
additional information enzyme is strictly specific for the beta-1,4-glucoside linkage exhibiting activity toward barley beta-glucan, lichenan, and carboxymethyl cellulose sodium salt, but not toward laminarin (1,3-beta-glucan), birchwood xylan, avicel, and not toward 4-nitrophenyl beta-D-glucoside or 4-nitrophenyl cellobioside. Cel6C cleaves the internal glycosidic linkages of cellooligosaccharides randomly, the predominant product of polysaccharide hydrolysis is cellobiose Humicola insolens ?
-
 ?
additional information enzyme is strictly specific for the beta-1,4-glucoside linkage exhibiting activity toward barley beta-glucan, lichenan, and carboxymethyl cellulose sodium salt, but not toward laminarin (1,3-beta-glucan), birchwood xylan, avicel, and not toward 4-nitrophenyl beta-D-glucoside or 4-nitrophenyl cellobioside. Cel6C cleaves the internal glycosidic linkages of cellooligosaccharides randomly, the predominant product of polysaccharide hydrolysis is cellobiose Humicola insolens Y1 ?
-
 ?

Synonyms

Synonyms Comment Organism
Cel6C
-
 Humicola insolens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Humicola insolens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable up to Humicola insolens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Humicola insolens

pH Range

pH Minimum pH Maximum Comment Organism
8
-
 98% of maximum activity Humicola insolens
9
-
 61% of maximum activity Humicola insolens
10
-
 27% of maximum activity Humicola insolens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 good stability Humicola insolens