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Literature summary for 3.2.1.4 extracted from
- Functional characterization and low-resolution structure of an endoglucanase Cel45A from the filamentous fungus Neurospora crassa OR74A: thermostable enzyme with high activity toward lichenan and beta-glucan (2015), Mol. Biotechnol., 57, 574-588.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 111% of initial activity | Neurospora crassa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31200 | - |
x * 31200, SDS-PAGE | Neurospora crassa |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | 20%, 122% of initial activity | Neurospora crassa |
| Ethanol | 20%, 119% of initial activity | Neurospora crassa |
| isopropanol | 20%, 119% of initial activity | Neurospora crassa |
| Methanol | 20%, 103% of initial activity | Neurospora crassa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | Q1K5M0 | - |
- |
| Neurospora crassa DSM 1257 | Q1K5M0 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.16 | - |
substrate phosphoric acid swollen cellulose, pH 6.0, 60°C | Neurospora crassa |
| 0.21 | - |
substrate carboxymethyl cellulose, pH 6.0, 60°C | Neurospora crassa |
| 0.54 | - |
substrate filter paper , pH 6.0, 60°C | Neurospora crassa |
| 1.02 | - |
substrate beta-glucan, pH 6.0, 60°C | Neurospora crassa |
| 1.05 | - |
substrate lichenan, pH 6.0, 60°C | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-glucan + H2O | - |
Neurospora crassa | ? | - |
? | |
| beta-glucan + H2O | - |
Neurospora crassa DSM 1257 | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Neurospora crassa | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Neurospora crassa DSM 1257 | ? | - |
? | |
| cellopentaose + H2O | minimal length substrate | Neurospora crassa | ? | - |
? | |
| cellopentaose + H2O | minimal length substrate | Neurospora crassa DSM 1257 | ? | - |
? | |
| filter paper + H2O | - |
Neurospora crassa | ? | - |
? | |
| lichenan + H2O | - |
Neurospora crassa | ? | - |
? | |
| lichenan + H2O | - |
Neurospora crassa DSM 1257 | ? | - |
? | |
| additional information | enzyme is most active against lichenan and beta-glucans and lesser active toward filter paper, carboxymethyl cellulose, and phosphoric acid-swollen cellulose | Neurospora crassa | ? | - |
? | |
| additional information | enzyme is most active against lichenan and beta-glucans and lesser active toward filter paper, carboxymethyl cellulose, and phosphoric acid-swollen cellulose | Neurospora crassa DSM 1257 | ? | - |
? | |
| phosphoric acid swollen cellulose + H2O | - |
Neurospora crassa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31200, SDS-PAGE | Neurospora crassa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cel45A | - |
Neurospora crassa |
| gh45-1 | - |
Neurospora crassa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Neurospora crassa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 72 | - |
melting temperature | Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Neurospora crassa |
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