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Literature summary for 3.2.1.4 extracted from
- Recombinant expression and characterization of a novel endoglucanase from Bacillus subtilis in Escherichia coli (2014), Mol. Biol. Rep., 41, 3295-3302.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Tween 20 | 150% of initial activity | Bacillus subtilis |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 70% of initial activity | Bacillus subtilis | |
| Cu2+ | 1 mM, 18% residual activity | Bacillus subtilis | |
| Hg2+ | 1 mM, 40% residual activity | Bacillus subtilis | |
| Ni2+ | 1 mM, 27% residual activity | Bacillus subtilis | |
| sodium dodecylsulfate | 7% residual activity | Bacillus subtilis | |
| Zn2+ | 1 mM, 21% residual activity | Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
carboxymethyl cellulose | Km-value 0.00176 mM, pH 9.0, 40°C | Bacillus subtilis |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| isopropanol | 130% of initial activity | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis JS2004 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl cellobioside + H2O | - |
Bacillus subtilis | 4-nitrophenol + cellobiose | - |
? | |
| 4-nitrophenyl cellobioside + H2O | - |
Bacillus subtilis JS2004 | 4-nitrophenol + cellobiose | - |
? | |
| avicel + H2O | - |
Bacillus subtilis | ? | - |
? | |
| avicel + H2O | - |
Bacillus subtilis JS2004 | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Bacillus subtilis | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Bacillus subtilis JS2004 | ? | - |
? | |
| additional information | no substrates: alpha-glucan, cellobiose and xylan | Bacillus subtilis | ? | - |
? | |
| additional information | no substrates: alpha-glucan, cellobiose and xylan | Bacillus subtilis JS2004 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 63000,SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 12 | more than 80% of maximum activity | Bacillus subtilis |
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