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Literature summary for 3.2.1.4 extracted from

  • Huy, N.D.1; Nguyen, C.L.; Park, H.S.; Loc, N.H.; Choi, M.S.; Kim, D.H.; Seo, J.W.; Park, S.M.
    Characterization of a novel manganese dependent endoglucanase belongs in GH family 5 from Phanerochaete chrysosporium (2016), J. Biosci. Bioeng., 121, 154-159.
    View publication on PubMed
Search for more literature for 3.2.1.4

Application

Application Comment Organism
degradation addition of Eg5A to cellobiase (i.e. cellobiohydrolase and beta-glucosidase) results in a 53% increasing saccharification of NaOH-pretreated barley straw, and the glucose release is 47% higher than with cellobiase treatment alone Phanerodontia chrysosporium
degradation addition of isoform Eg5A to cellobiase (cellobiohydrolase and beta-glucosidase) results in a 53% increasing saccharification of NaOH-pretreated barley straw, whereas the glucose release is 47% higher than that cellobiase treatment alone Phanerodontia chrysosporium

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
 carboxymethyl cellulose pH 5.0, 50°C Phanerodontia chrysosporium

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0 mM, 177% of initial activity Phanerodontia chrysosporium
Mn2+ 20 mM, 177% of initial activity Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE and calculated Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
-
-
Phanerodontia chrysosporium W5ZNX7
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
622
-
 substrate glucomannan, pH 5.0, 50°C Phanerodontia chrysosporium
694
-
 substrate carboxymethyl cellulose, pH 5.0, 50°C Phanerodontia chrysosporium
1443
-
 substrate beta-glucan, pH 5.0, 50°C Phanerodontia chrysosporium
1444
-
 substrate barley beta-glucan, pH 5.0, 50°C Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
 Phanerodontia chrysosporium ?
-
 ?
beta-glucan + H2O
-
 Phanerodontia chrysosporium ?
-
 ?
carboxymethyl cellulose + H2O
-
 Phanerodontia chrysosporium ?
-
 ?
glucomannan + H2O
-
 Phanerodontia chrysosporium ?
-
 ?
additional information amino acids E209 and E319 act as proton donor and nucleophile in substrate catalytic domain Phanerodontia chrysosporium ?
-
 ?
additional information very poor or no substrates: laminarin, xyloglucan, birchwood xylan, arabinan, arabinoxylan, 4-nitrophenyl-beta-D-glucoside. Amino acids E209 and E319 act as proton donor and nucleophile in the catalytic domain Phanerodontia chrysosporium ?
-
 ?
additional information very poor substrates: laminarin, xyloglucan. No substrates: birchwood xylan, arabinan, arabinoxylan, 4-nitrophenyl-beta-D-glucopyranoside Phanerodontia chrysosporium ?
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE and calculated Phanerodontia chrysosporium

Synonyms

Synonyms Comment Organism
Eg5a
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60
-
 Phanerodontia chrysosporium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 more than 50% of maximum activity Phanerodontia chrysosporium
20
-
 50% of maximum activity Phanerodontia chrysosporium
80
-
 50% of maximum activity Phanerodontia chrysosporium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 12 h, stable Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Phanerodontia chrysosporium
4.5 6
-
 Phanerodontia chrysosporium

pH Range

pH Minimum pH Maximum Comment Organism
3 8
-
 Phanerodontia chrysosporium