Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.4 extracted from

  • Kim, S.J.; Joo, J.E.; Jeon, S.D.; Hyeon, J.E.; Kim, S.W.; Um, Y.S.; Han, S.O.
    Enhanced thermostability of mesophilic endoglucanase Z with a high catalytic activity at active temperatures (2016), Int. J. Biol. Macromol., 86, 269-276.
    View publication on PubMed
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Clostridium cellulovorans

Protein Variants

Protein Variants Comment Organism
K94R increase in specific activity Clostridium cellulovorans
K94R/S365P optimal temperature of K94R/S365P is increased by 7.5°C compared to wild-type. K94R/S365P retains 78.3% relative activity at 70°C, while the wild-type retains 5.8%. K94R/S365P shows 45.1fold higher activity than the wild-type at 70°C and 3.1fold higher activity at 42.5°C, which is the optimal temperature ofthe wild type. K94R/S365P is stimulated in 2.5fold lower concentration of CaCl2 and displays delayed aggregation temperature in the presence of CaCl2 compared to the wild type. In long-term hydrolysis, K94R/S365P reduces the newly released reducing sugars after 12 h reaction Clostridium cellulovorans
S365P increase in specific activity Clostridium cellulovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 carboxymethyl cellulose Km-values for wild-type, mutants K94R, S365P and K94R/S365P are 7.2, 3.7, 6.4 and 5.0 mg/ml, respectively, pH 7.0, temperature not specified in the publication Clostridium cellulovorans

Organism

Organism UniProt Comment Textmining
Clostridium cellulovorans D9SRK9
-
-
Clostridium cellulovorans DSM 3052 D9SRK9
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.25
-
 wild-type, pH 7.0, 60°C Clostridium cellulovorans
0.33
-
 mutant S365P, pH 7.0, 60°C Clostridium cellulovorans
0.33
-
 wild-type, pH 7.0, 40°C Clostridium cellulovorans
0.39
-
 mutant S365P, pH 7.0, 40°C Clostridium cellulovorans
0.44
-
 mutant K94R, pH 7.0, 60°C Clostridium cellulovorans
0.45
-
 mutant K94R, pH 7.0, 40°C Clostridium cellulovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
 Clostridium cellulovorans ?
-
 ?
carboxymethyl cellulose + H2O
-
 Clostridium cellulovorans DSM 3052 ?
-
 ?

Synonyms

Synonyms Comment Organism
Clocel_2741
-
 Clostridium cellulovorans
EngZ
-
 Clostridium cellulovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
43.9
-
 melting temperature, mutant K94R/S365P Clostridium cellulovorans
47.3
-
 melting temperature, mutant K94R Clostridium cellulovorans
48.9
-
 melting temperature, wild-type Clostridium cellulovorans
49.1
-
 melting temperature, mutant S365P Clostridium cellulovorans