Any feedback?
Literature summary for 3.2.1.4 extracted from
- Purification and characterization of an acidothermophilic cellulase enzyme produced by Bacillus subtilis strain LFS3 (2012), Extremophiles, 16, 637-644.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | 5 mM, 5% residual activity | Bacillus subtilis |  |
| Hg2+ | 5 mM, 10% residual activity | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Na+ | 5 mM, 130% of initial activity | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 185000 | - |
PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis LFS3 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.4 | - |
pH 4.0, 60°C | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl cellulose + H2O | - |
Bacillus subtilis | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Bacillus subtilis LFS3 | ? | - |
? | |
| filter paper + H2O | 8.4% of the activity with carboxymethyl cellulose | Bacillus subtilis | ? | - |
? | |
| filter paper + H2O | 8.4% of the activity with carboxymethyl cellulose | Bacillus subtilis LFS3 | ? | - |
? | |
| additional information | no substrates: 4-nitrophenyl beta-D-glucopyranoside, cellulose | Bacillus subtilis | ? | - |
? | |
| additional information | no substrates: 4-nitrophenyl beta-D-glucopyranoside, cellulose | Bacillus subtilis LFS3 | ? | - |
? | |
| xylan + H2O | 20.3% of the activity with carboxymethyl cellulose | Bacillus subtilis | ? | - |
? | |
| xylan + H2O | 20.3% of the activity with carboxymethyl cellulose | Bacillus subtilis LFS3 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
80% of maximum activity | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
30 min, 29% residual activity | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | more than 75% of maximum activity | Bacillus subtilis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
