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Literature summary for 3.2.1.4 extracted from

  • Zhang, G.; Li, S.; Xue, Y.; Mao, L.; Ma, Y.
    Effects of salts on activity of halophilic cellulase with glucomannanase activity isolated from alkaliphilic and halophilic Bacillus sp. BG-CS10 (2012), Extremophiles, 16, 35-43.
    View publication on PubMed
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 5 mM, 37% residual activity Bacillus sp. (in: Bacteria)
Hg2+ 5 mM, 45% residual activity Bacillus sp. (in: Bacteria)
Mg2+ 5 mM, 44% residual activity Bacillus sp. (in: Bacteria)
Ni2+ 5 mM, 52% residual activity Bacillus sp. (in: Bacteria)
Zn2+ 5 mM, 19% residual activity Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 carboxymethyl cellulose Km value is 6.6 mg/ml in absence, 3.18 mg/ml in presence of 2.5 M NaCl, respectively, at pH 5.0, 55°C Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
KCl enzyme activity increases approximately tenfold with 2.5 M NaCl or 3 M KCl Bacillus sp. (in: Bacteria)
LiCl 5 mM, 112% of initial activity activity Bacillus sp. (in: Bacteria)
NaCl enzyme activity increases approximately tenfold with 2.5 M NaCl or 3 M KCl Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 x * 62000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) D4P8C6
-
-
Bacillus sp. (in: Bacteria) BG-CS10 D4P8C6
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33
-
 presence of 2.5 M NaCl, pH 5.0, 55°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
carboxymethyl cellulose + H2O
-
 Bacillus sp. (in: Bacteria) BG-CS10 ?
-
 ?
konac glucomannan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
konac glucomannan + H2O
-
 Bacillus sp. (in: Bacteria) BG-CS10 ?
-
 ?
additional information enzyme hydrolyzes soluble cellulose substrates containing beta-1,4-linkages, such as carboxylmethyl cellulose and konjac glucomannan, but has no exoglucanase and beta-glucosidase activities Bacillus sp. (in: Bacteria) ?
-
 ?
additional information enzyme hydrolyzes soluble cellulose substrates containing beta-1,4-linkages, such as carboxylmethyl cellulose and konjac glucomannan, but has no exoglucanase and beta-glucosidase activities Bacillus sp. (in: Bacteria) BG-CS10 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 62000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Bacillus sp. (in: Bacteria)
55
-
 presence of 2.5 M NaCl Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 30 min, complete loss of activity in absence of NaCl, no loss in presence of 2.5 M NaCl Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26
-
 carboxymethyl cellulose presence of 2.5 M NaCl, pH 5.0, 55°C Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9.2 presence of 2.5 M NaCl Bacillus sp. (in: Bacteria)