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Literature summary for 3.2.1.4 extracted from

  • Wang, K.; Luo, H.; Bai, Y.; Shi, P.; Huang, H.; Xue, X.; Yao, B.
    A thermophilic endo-1,4-beta-glucanase from Talaromyces emersonii CBS394.64 with broad substrate specificity and great application potentials (2014), Appl. Microbiol. Biotechnol., 98, 7051-7060.
    View publication on PubMed
Search for more literature for 3.2.1.4

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 5 mM, 174% of initial activity Rasamsonia emersonii
sodium dodecylsulfate 5 mM, 110% of initial activity Rasamsonia emersonii

Application

Application Comment Organism
degradation under simulated mashing conditions, addition of 60 U Egl5A reduces more viscosity (10.0 vs.7.6%) than 80 U of Ultraflo XL from Novozymes Rasamsonia emersonii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Rasamsonia emersonii

Inhibitors

Inhibitors Comment Organism Structure
Cr3+ 5 mM, 34% residual activity Rasamsonia emersonii
Cu2+ 5 mM, 41% residual activity Rasamsonia emersonii
EDTA 5 mM, 46% residual activity Rasamsonia emersonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 Barley beta-glucan Km value 12.2 mg/l, pH 4.5, 90°C Rasamsonia emersonii
additional information
-
 carboxymethyl cellulose Km value 31.7 mg/l, pH 4.5, 90°C Rasamsonia emersonii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM, 128% of initial activity Rasamsonia emersonii
K+ 5 mM, 137% of initial activity Rasamsonia emersonii
Li+ 5 mM, 150% of initial activity Rasamsonia emersonii
Zn2+ 5 mM, 142% of initial activity Rasamsonia emersonii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36800
-
 x * 36800, calculated, x * 37000, SDS-PAGE of deglycosylated enzyme Rasamsonia emersonii
37000
-
 x * 36800, calculated, x * 37000, SDS-PAGE of deglycosylated enzyme Rasamsonia emersonii

Organism

Organism UniProt Comment Textmining
Rasamsonia emersonii X4YXV3
-
-
Rasamsonia emersonii CBS394.64 X4YXV3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme migrates on SDS-PAGE with several bands, with molecular masses larger than its theoretical value. after Endo H-treatment, Egl5A migrates as a single band of 37000 Da Rasamsonia emersonii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.3
-
 substrate birchwood xylan, pH 4.5, 90°C Rasamsonia emersonii
176
-
 substrate carob bean gum, pH 4.5, 90°C Rasamsonia emersonii
624
-
 substrate carboxymethyl cellulose, pH 4.5, 90°C Rasamsonia emersonii
3413
-
 substrate lichenan, pH 4.5, 90°C Rasamsonia emersonii
4059
-
 substrate laminarin, pH 4.5, 90°C Rasamsonia emersonii
4254
-
 substrate barley beta-glucan, pH 4.5, 90°C Rasamsonia emersonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
 Rasamsonia emersonii ?
-
 ?
birchwood xylan + H2O
-
 Rasamsonia emersonii ?
-
 ?
carboxymethyl cellulose + H2O
-
 Rasamsonia emersonii ?
-
 ?
carob bean gum + H2O
-
 Rasamsonia emersonii ?
-
 ?
laminarin + H2O
-
 Rasamsonia emersonii ?
-
 ?
lichenan + H2O
-
 Rasamsonia emersonii ?
-
 ?
additional information enzyme displays broad substrate specificity and exhibits high activity on substrates containing beta-1,4-glycosidic bonds and beta-1,3-glycosidic bonds such as barley beta-glucan, laminarin, lichenan, carboxymethyl cellulose, carob bean gum, and birchwood xylan Rasamsonia emersonii ?
-
 ?
additional information no substrates: avicel, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-cellobioside Rasamsonia emersonii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 36800, calculated, x * 37000, SDS-PAGE of deglycosylated enzyme Rasamsonia emersonii

Synonyms

Synonyms Comment Organism
Egl5a
-
 Rasamsonia emersonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Rasamsonia emersonii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80 95 more than 70% of maximum activity Rasamsonia emersonii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 1 h, 50% residual activity Rasamsonia emersonii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
51.6
-
 carboxymethyl cellulose pH 4.5, 90°C Rasamsonia emersonii
311
-
 Barley beta-glucan pH 4.5, 90°C Rasamsonia emersonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Rasamsonia emersonii

pH Range

pH Minimum pH Maximum Comment Organism
4 5 more than 90% of maximum activity Rasamsonia emersonii

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 10 37°C, 1 h, more than than 80% of maximum activity Rasamsonia emersonii