Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Benzene | enhances enzyme activity | Haloarcula sp. | |
n-hexane | enhances enzyme activity | Haloarcula sp. |
Application | Comment | Organism |
---|---|---|
biotechnology | considering its thermostable, alkali-stable, halostable and organic solvent-tolerant properties, the enzyme might be potentially useful for future applications in biotechnological processes | Haloarcula sp. |
General Stability | Organism |
---|---|
halostable, the enzyme retains more than 80% activity after incubation with 1030% NaCl for 24 h | Haloarcula sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl dicarbonate | 10 mM, 17.6% residual activity | Haloarcula sp. | |
EDTA | 10 mM, 11.2% residual activity | Haloarcula sp. | |
Hg2+ | 5 mM, 58% inhibition | Haloarcula sp. | |
phenylmethylsulfonyl fluoride | 10 mM, 18.1% residual activity | Haloarcula sp. | |
SDS | 10 mM, 49.2% residual activity | Haloarcula sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
cellulose | Km-value for carboxymethylcellulose-Na as substrate is 3.01 mg/ml | Haloarcula sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Haloarcula sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 1.3fold stimulation | Haloarcula sp. | |
Mg2+ | 5 mM, 1.5fold stimulation | Haloarcula sp. | |
NaCl | optimal activity at 17.5% NaCl | Haloarcula sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
gel filtration | Haloarcula sp. |
Organic Solvent | Comment | Organism |
---|---|---|
1-butanol | decreases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
1-decanol | increases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
Acetone | decreases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
acetonitrile | decreases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
benzene | increases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
chloroform | no effect on stability | Haloarcula sp. |
cyclohexane | increases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
DMSO | decreases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
Ethanol | decreases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
isooctane | increases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
Methanol | decreases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
additional information | high activity and stability in the presence of non-polar hydrophobic organic solvents | Haloarcula sp. |
n-hexane | increases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
phenylmethylsulfonyl fluoride | decreases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
toluene | increases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloarcula sp. | - |
- |
- |
Haloarcula sp. G10 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloarcula sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
71.2 | - |
pH 9.0, 50°C | Haloarcula sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellulose + H2O | - |
Haloarcula sp. | ? | - |
? | |
cellulose + H2O | - |
Haloarcula sp. G10 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, SDS-PAGE | Haloarcula sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Haloarcula sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | 20°C: about 40% of maximal activity, 80°C: about 80% of maximal activity | Haloarcula sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
24 h incubation, enzyme retains more than 60% activity | Haloarcula sp. |
90 | - |
24 h incubation, complete loss of activity | Haloarcula sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Haloarcula sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | pH 6.0: about 80% of maximal activity, pH 11: about 70% of maximal activity | Haloarcula sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | good stability in the pH range from7.011.0 | Haloarcula sp. |