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Literature summary for 3.2.1.4 extracted from

  • Ando, S.; Ishida, H.; Kosugi, Y.; Ishikawa, K.
    Hyperthermostable endoglucanase from Pyrococcus horikoshii (2002), Appl. Environ. Microbiol., 68, 430-433.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
industry because of its ability to hydrolyze celluloses at high temperatures above 90°C, as well as its thermostability, the enzyme is expected to be an excellent tool for industrial hydrolysis of cellulose, particularly for biopolishing of cotton products Pyrococcus horikoshii

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000, SDS-PAGE Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58925
-
-
Pyrococcus horikoshii DSM 12428 O58925
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
Pyrococcus horikoshii ?
-
?
avicel + H2O
-
Pyrococcus horikoshii DSM 12428 ?
-
?
carboxymethyl cellulose + H2O
-
Pyrococcus horikoshii ?
-
?
carboxymethyl cellulose + H2O
-
Pyrococcus horikoshii DSM 12428 ?
-
?
lichenan + H2O
-
Pyrococcus horikoshii ?
-
?
lichenan + H2O
-
Pyrococcus horikoshii DSM 12428 ?
-
?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
PH1171 locus name Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
assay at Pyrococcus horikoshii
97
-
-
Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
97
-
3 h, 20% loss of activity Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
assay at Pyrococcus horikoshii