Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.4 extracted from

  • Girfoglio, M.; Rossi, M.; Cannio, R.
    Cellulose degradation by Sulfolobus solfataricus requires a cell-anchored endo-beta-1-4-glucanase (2012), J. Bacteriol., 194, 5091-5100.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloning and expression of the sso1354 gene in different hosts, Escherichia coli and Sulfolobus solfataricus itself Saccharolobus solfataricus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Saccharolobus solfataricus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35154
-
x * 35154, enzyme recombinantly expressed in Escherichia coli, calculated from sequence Saccharolobus solfataricus
35500
-
x * 35500, enzyme recombinantly expressed in Escherichia coli, SDS-PAGE Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97YG7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
pH 5.0, specific activity of the enzyme recombinantly expressed in Sulfolobus solfataricus is about 200fold higher compared to the specific activity of the enzyme recombinantly expressed in Escherichia coli Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl-cellulose + H2O
-
Saccharolobus solfataricus ?
-
?
cellulose + H2O
-
Saccharolobus solfataricus ?
-
?
curdlan + H2O activity is 26% compared to activity with carboxymethyl-cellulose Saccharolobus solfataricus ?
-
?
debranched arabinan activity is 25% compared to activity with carboxymethyl-cellulose Saccharolobus solfataricus ?
-
?
lichenan + H2O activity is 120% compared to activity with carboxymethyl-cellulose Saccharolobus solfataricus ?
-
?
additional information no hydrolysis of p-nitrophenyl-beta-D-cellobioside and p-nitrophenyl-beta-D-cellotrioside, no exocellulase activity Saccharolobus solfataricus ?
-
?
oat spelt xylan + H2O activity is 25% compared to activity with carboxymethyl-cellulose Saccharolobus solfataricus ?
-
?
pachyman + H2O activity is 22% compared to activity with carboxymethyl-cellulose Saccharolobus solfataricus ?
-
?

Subunits

Subunits Comment Organism
? x * 35154, enzyme recombinantly expressed in Escherichia coli, calculated from sequence Saccharolobus solfataricus
? x * 35500, enzyme recombinantly expressed in Escherichia coli, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
SSO1354 enzyme
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
enzyme recombinantly expressed in Sulfolobus solfataricus Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
half-life: 180 min Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
recombinant enzyme from Sulfolobus solfataricus Saccharolobus solfataricus

General Information

General Information Comment Organism
physiological function the enzyme is responsible for the main cellulase activity of Sulfolobus solfataricus Saccharolobus solfataricus