Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.4 extracted from

  • Bose, S.; Barnes, C.A.; Petrich, J.W.
    Enhanced stability and activity of cellulase in an ionic liquid and the effect of pretreatment on cellulose hydrolysis (2011), Biotechnol. Bioeng., 109, 434-443.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information pretreatment of cellulose with ionic liquids such as 1-butyl-3-methylimidazolium chloride, 1-methylimidazolium chloride, and tris-(2-hydroxyethyl)-methylammonium methylsulfate results in more rapid conversion to glucose than hydrolysis with cellulose that is not pretreated Aspergillus niger
tris-(2-hydroxyethyl)-methylammonium methylsulfate enzymic activity is significantly enhanced in the presence of tris-(2-hydroxyethyl)-methylammonium methylsulfate in buffer. Tris-(2-hydroxyethyl)-methylammonium methylsulfate imparts substantial thermostability to the enzyme Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
1-butyl-3-methylimidazolium methylsulfate the enzyme precipitates immediately on addition to the reaction mixture Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Aspergillus niger
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellulose + H2O pretreatment of cellulose with ionic liquids such as 1-butyl-3-methylimidazolium chloride, 1-methylimidazolium chloride, and tris-(2-hydroxyethyl)-methylammonium methylsulfate results in more rapid conversion to glucose than hydrolysis with cellulose that is not pretreated Aspergillus niger ?
-
?

Synonyms

Synonyms Comment Organism
endo-1,4-beta-D-glucanase
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
the percent conversion to glucose from pretreated cellulose is increased when the temperature is increased from 45 to 60°C Aspergillus niger
45
-
-
Aspergillus niger
75
-
presence of tris-(2-hydroxyethyl)-methylammonium methylsulfate Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 75 activity range Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
inactivation Aspergillus niger
55
-
denaturation above Aspergillus niger
75
-
denaturation, presence of tris-(2-hydroxyethyl)-methylammonium methylsulfate Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
assay at Aspergillus niger

General Information

General Information Comment Organism
additional information effects of different solvents, ions, and viscosities on enzyme activity at different temperatures, overview Aspergillus niger