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Literature summary for 3.2.1.4 extracted from

  • Ahmed, S.; Deka, D.; Jawed, M.; Goyal, D.; Fontes, C.; Goyal, A.
    Biochemical characterization of a recombinant derivative (CtLic26A-Cel5) of a cellulosomal cellulase from Clostridium thermocellum (2009), Curr. Trends Biotechnol. Pharm., 3, 56-63.
No PubMed abstract available
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged wild-type and truncated mutant cellulase in Escherichia coli strain BL21 Acetivibrio thermocellus

Protein Variants

Protein Variants Comment Organism
additional information construction of a His6-tagged truncated enzyme mutant CtLic26A-Cel5 Acetivibrio thermocellus

Localization

Localization Comment Organism GeneOntology No. Textmining
cellulosome
-
 Acetivibrio thermocellus 43263
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates the truncated enzyme mutant CtLic26A-Cel5 about 2fold Acetivibrio thermocellus
Mg2+ activates the truncated enzyme mutant CtLic26A-Cel5 about 2fold Acetivibrio thermocellus
Na+ activates the truncated enzyme mutant CtLic26A-Cel5 about 2fold Acetivibrio thermocellus
Ni2+ activates the truncated enzyme mutant CtLic26A-Cel5 about 2fold Acetivibrio thermocellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 x * 64000, recombinant truncated mutant, SDS-PAGE Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged truncated mutant cellulase CtLic26A-Cel5 from Escherichia coli strain BL21 by metal affinity chromatography Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrates are acid swollen cellulose, lichenan, beta-glucan, carboxymethyl cellulose, galactomannans, oat spelt xylan, avicel, and steam exploded bagasse, no activity glucomannan and laminarin Acetivibrio thermocellus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 64000, recombinant truncated mutant, SDS-PAGE Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 truncated enzyme mutant CtLic26A-Cel5 Acetivibrio thermocellus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 90 temperature profile Acetivibrio thermocellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified recombinant mutant CtLic26A-Cel5, maximally stable Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3
-
 truncated enzyme mutant CtLic26A-Cel5 Acetivibrio thermocellus

pH Range

pH Minimum pH Maximum Comment Organism
3 8 pH profile Acetivibrio thermocellus