Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
SDS-PAGE | Cherax destructor |
52000 | - |
- |
Gecarcoidea natalis |
52000 | - |
SDS-PAGE | Gecarcoidea natalis |
52000 | - |
endo-beta-1,4-glucanase 2 | Cherax destructor |
53000 | - |
SDS-PAGE | Cherax destructor |
53000 | - |
endo-beta-1,4-glucanase 1 | Cherax destructor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cherax destructor | - |
- |
- |
Cherax destructor Clark 1936 | - |
- |
- |
Gecarcoidea natalis | - |
- |
- |
Gecarcoidea natalis Pocock 1888 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Cherax destructor |
gel filtration | Gecarcoidea natalis |
precipitation, a combination of anion exchange, hydrophobic interaction and gel filtration chromatography, all chromatography is carried out at 4°C | Cherax destructor |
precipitation, a combination of anion exchange, hydrophobic interaction and gel filtration chromatography, all chromatography is carried out at 4°C | Gecarcoidea natalis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
midgut | - |
Cherax destructor | - |
midgut | - |
Gecarcoidea natalis | - |
midgut gland | - |
Cherax destructor | - |
midgut gland | - |
Gecarcoidea natalis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.023 | - |
homogenate | Gecarcoidea natalis |
0.166 | - |
after DEAE anion exchange chromatography | Gecarcoidea natalis |
0.23 | - |
homogenate | Cherax destructor |
5.13 | - |
after DEAE anion exchange chromatography | Cherax destructor |
7.43 | - |
after gel filtration chromatography | Cherax destructor |
11.61 | - |
after DEAE anion exchange chromatography | Cherax destructor |
12.74 | - |
last purification step | Gecarcoidea natalis |
12.74 | - |
after gel filtration chromatography | Gecarcoidea natalis |
19.09 | - |
last purification step | Cherax destructor |
19.09 | - |
after gel filtration chromatography | Cherax destructor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + H2O | - |
Cherax destructor | D-glucose + cellobiose + cellooligosaccharide | - |
? | |
carboxymethyl cellulose + H2O | - |
Cherax destructor Clark 1936 | D-glucose + cellobiose + cellooligosaccharide | - |
? | |
carboxymethyl cellulose + H2O | - |
Gecarcoidea natalis | glucose + cellobiose + short oligomers | - |
? | |
carboxymethyl cellulose + H2O | - |
Gecarcoidea natalis Pocock 1888 | glucose + cellobiose + short oligomers | - |
? | |
carboxymethyl cellulose + H2O | - |
Cherax destructor | additional information | short glucose oligomers, cellobiose and a very small amount of glucose | ? | |
carboxymethyl cellulose + H2O | - |
Cherax destructor Clark 1936 | additional information | short glucose oligomers, cellobiose and a very small amount of glucose | ? | |
carboxymethylcellulose + H2O | - |
Gecarcoidea natalis | short glucose oligomers + cellobiose + glucose | - |
? | |
carboxymethylcellulose + H2O | - |
Gecarcoidea natalis Pocock 1888 | short glucose oligomers + cellobiose + glucose | - |
? | |
additional information | glucanase hydrolyses beta-1,4-glycosidic bonds | Cherax destructor | ? | - |
? | |
additional information | glucanase hydrolyses mainly beta-1,4-glycosidic bonds but is also capable of significant hydrolysis of beta-1,3-glycosidic bonds | Gecarcoidea natalis | ? | - |
? | |
additional information | glucanase hydrolyses mainly beta-1,4-glycosidic bonds but is also capable of significant hydrolysis of beta-1,3-glycosidic bonds | Gecarcoidea natalis Pocock 1888 | ? | - |
? | |
additional information | glucanase hydrolyses beta-1,4-glycosidic bonds | Cherax destructor Clark 1936 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo-1,4-beta-glucanase 1 | - |
Cherax destructor |
endo-1,4-beta-glucanase 2 | - |
Cherax destructor |
endo-beta-1,4-glucanase | - |
Gecarcoidea natalis |
endo-beta-1,4-glucanase 1 | - |
Cherax destructor |
endo-beta-1,4-glucanase 2 | - |
Cherax destructor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | - |
Gecarcoidea natalis |
5.5 | - |
endo-beta-1,4-glucanase 1 | Cherax destructor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | - |
Gecarcoidea natalis |