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Literature summary for 3.2.1.4 extracted from
- Differential cellulolytic activity of native-form and C-terminal tagged-form cellulase derived from coptotermes formosanus and expressed in E. coli (2009), Insect Biochem. Mol. Biol., 39, 516-522.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned and overexpressed in both native form (nCfEG) and C-terminal His-tagged form (tCfEG) in Escherichia coli | Coptotermes formosanus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | C-terminal His-tagged form (tCfEG) shows hydrolytic activity on cellulosic substrates | Coptotermes formosanus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47090 | - |
calculated molecular weight, C-terminal His-tagged form | Coptotermes formosanus |
| 48160 | - |
calculated molecular weight, wild-type | Coptotermes formosanus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coptotermes formosanus | Q9BLD2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified to near homogeneity, gel filtration | Coptotermes formosanus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl cellulose + H2O | - |
Coptotermes formosanus | ? | - |
? |  |
| cellobiose + H2O | - |
Coptotermes formosanus | ? | - |
? | |
| cellotetraose + H2O | - |
Coptotermes formosanus | ? | - |
? | |
| cellotriose + H2O | - |
Coptotermes formosanus | ? | - |
? | |
| additional information | hydrolyzing internal beta-1,4-glycosidic bonds and resulting in a smear of polymers with different lengths. The hydrolytic products of tCfEG are one unit sugar less than those produced by nCfEG | Coptotermes formosanus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cellulase | - |
Coptotermes formosanus |
| CfEG3a | - |
Coptotermes formosanus |
| endoglucanase | - |
Coptotermes formosanus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Coptotermes formosanus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 52 | - |
Coptotermes formosanus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Coptotermes formosanus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Coptotermes formosanus |
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Release 2023.1 (January 2023)
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