Application | Comment | Organism |
---|---|---|
biotechnology | Production of immobilized enzyme on Sepabeads EC-BU (hydrophobic interactions are the driving force for the adsorption of the enzyme to the carrier) | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
The gene encoding for the thermoactive endoglucanase is derived from a metagenomic gene library based on a mixed culture grown anaerobically on cellulose at 70 C and pH 7. The recombinant endoglucanase is actively expressed in Escherichia coli TunerTM(DE3)pLacI by induction with 1 mM IPTG. | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
- |
Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas putida | beta-1,4-endoglucanases show activities of the cleavage of the cellulose backbone due to an endocleavage. Oligosaccharides of different lengths as well as cellobiose and glucose are formed | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by Ni-NTA column purification and pretreatment by heat denaturation to remove accompanying proteins | Pseudomonas putida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | metagenome | Pseudomonas putida | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | beta-1,4-endoglucanases show activities of the cleavage of the cellulose backbone due to an endocleavage. Oligosaccharides of different lengths as well as cellobiose and glucose are formed | Pseudomonas putida | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endoglucanase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
enzyme is thermoactive | Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
free enzyme shows an activity of 75% of the initial activity after 30 min, and even an activity of 40% remained after 6 h, both measured at 70°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pseudomonas putida |