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Literature summary for 3.2.1.4 extracted from

  • Zheng, B.; Yang, W.; Wang, Y.; Feng, Y.; Lou, Z.
    Crystallization and preliminary crystallographic analysis of thermophilic cellulase from Fervidobacterium nodosum Rt17-B1 (2009), Acta Crystallogr. Sect. F, 65, 219-222.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Fervidobacterium nodosum Rt17-B1

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour-diffusion method Fervidobacterium nodosum Rt17-B1

Organism

Organism UniProt Comment Textmining
Fervidobacterium nodosum Rt17-B1 A7HLZ2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-chelating affinity column, SDS-PAGE Fervidobacterium nodosum Rt17-B1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O high specific activity towards carboxymethylcellulose Fervidobacterium nodosum Rt17-B1 ?
-
 ?
cellulose + H2O
-
 Fervidobacterium nodosum Rt17-B1 D-glucose + ?
-
 ?

Synonyms

Synonyms Comment Organism
Cellulase
-
 Fervidobacterium nodosum Rt17-B1
endoglucanase
-
 Fervidobacterium nodosum Rt17-B1
FnCel5A
-
 Fervidobacterium nodosum Rt17-B1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
79.8
-
-
 Fervidobacterium nodosum Rt17-B1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 high thermostability (Topt: 79.8°C, half-life 48 h) Fervidobacterium nodosum Rt17-B1