Cloned (Comment) | Organism |
---|---|
wild-type enzyme and mutant enzymes E186Q and E359Q, expression in Escherichia coli | Acetivibrio thermocellus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method at 20°C, crystal structure of Cel44A is solved at a resolution of 0.96 A.The crystal structures of E186Q mutant complexed with cellopentaose and cellohexaose are solved at 2.0 and 1.8 A resolution, respectively | Acetivibrio thermocellus |
Protein Variants | Comment | Organism |
---|---|---|
E186Q | mutant has no cellulase activity | Acetivibrio thermocellus |
E359Q | mutant has no cellulase activity | Acetivibrio thermocellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethylcellulose + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
cellohexaitol + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
cellohexaose + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
cellopentaose + H2O | - |
Acetivibrio thermocellus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cel44A | - |
Acetivibrio thermocellus |