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Literature summary for 3.2.1.4 extracted from

  • Kitago, Y.; Karita, S.; Watanabe, N.; Kamiya, M.; Aizawa, T.; Sakka, K.; Tanaka, I.
    Crystal structure of Cel44A, a glycoside hydrolase family 44 endoglucanase from Clostridium thermocellum (2007), J. Biol. Chem., 282, 35703-35711.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type enzyme and mutant enzymes E186Q and E359Q, expression in Escherichia coli Acetivibrio thermocellus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method at 20°C, crystal structure of Cel44A is solved at a resolution of 0.96 A.The crystal structures of E186Q mutant complexed with cellopentaose and cellohexaose are solved at 2.0 and 1.8 A resolution, respectively Acetivibrio thermocellus

Protein Variants

Protein Variants Comment Organism
E186Q mutant has no cellulase activity Acetivibrio thermocellus
E359Q mutant has no cellulase activity Acetivibrio thermocellus

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O
-
Acetivibrio thermocellus ?
-
?
cellohexaitol + H2O
-
Acetivibrio thermocellus ?
-
?
cellohexaose + H2O
-
Acetivibrio thermocellus ?
-
?
cellopentaose + H2O
-
Acetivibrio thermocellus ?
-
?

Synonyms

Synonyms Comment Organism
Cel44A
-
Acetivibrio thermocellus