Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, 1.2fold activation | Lysobacter sp. | |
NEM | 1 mM, 1.4fold activation | Lysobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide | 100 mM, 98% inhibition | Lysobacter sp. | |
Hg2+ | 1 mM, complete inhibition | Lysobacter sp. | |
Monoiodoacetic acid | 1 mM, 10% inhibition | Lysobacter sp. | |
N-bromosuccinimide | 1 mM, 99% inhibition | Lysobacter sp. | |
o-phenanthroline | 1 mM, 75% inhibition | Lysobacter sp. | |
p-chloromercuribenzoate | 0.01 mM, 20% inhibition | Lysobacter sp. | |
PMSF | 1 mM, 89% inhibition | Lysobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM, 1.3fold activation | Lysobacter sp. | |
Ca2+ | 1 mM, 1.3fold activation | Lysobacter sp. | |
Mg2+ | 1 mM, 1.3fold activation | Lysobacter sp. | |
Zn2+ | 1 mM, 1.3fold activation | Lysobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
x * 41000, SDS-PAGE | Lysobacter sp. |
41241 | - |
x * 41241, calculated from sequuence | Lysobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysobacter sp. | Q25C15 | - |
- |
Lysobacter sp. IB-9374 | Q25C15 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Lysobacter sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Lysobacter sp. | - |
Storage Stability | Organism |
---|---|
4°C, pH 6.5, 24 h , stable | Lysobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + H2O | - |
Lysobacter sp. | cellobiose + ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Lysobacter sp. IB-9374 | cellobiose + ? | - |
? | |
cellohexaose + H2O | major product is cellotriose, minor products are cellobiose and cellotetraose | Lysobacter sp. | cellotriose + cellotetraose + cellobiose | - |
? | |
cellohexaose + H2O | major product is cellotriose, minor products are cellobiose and cellotetraose | Lysobacter sp. IB-9374 | cellotriose + cellotetraose + cellobiose | - |
? | |
additional information | the enzyme also shows chitosanase activity. Colloidal chitosan, chitosan and glycol chitosan are hydrolyzed by Cel8A at 15-40% the activity of cellulose | Lysobacter sp. | ? | - |
? | |
additional information | the enzyme also shows chitosanase activity. Colloidal chitosan, chitosan and glycol chitosan are hydrolyzed by Cel8A at 15-40% the activity of cellulose | Lysobacter sp. IB-9374 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41000, SDS-PAGE | Lysobacter sp. |
? | x * 41241, calculated from sequuence | Lysobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
Cel8A | - |
Lysobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Lysobacter sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | 20°C: about 55% of maximal activity, 60°C: about 60% of maximal activity | Lysobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to | Lysobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
cellulase activity | Lysobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6.5 | pH 4.5: about 85% of maximal activity, pH 6.5: about 40% of maximal activity | Lysobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | 24 h, 4°C, more than 80% of original activity is retained | Lysobacter sp. |
6.5 | - |
24 h, 4°C, most stable at | Lysobacter sp. |