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Literature summary for 3.2.1.4 extracted from

  • Ogura, J.; Toyoda, A.; Kurosawa, T.; Chong, A.L.; Chohnan, S.; Masaki, T.
    Purification, characterization, and gene analysis of cellulase (Cel8A) from Lysobacter sp. IB-9374 (2006), Biosci. Biotechnol. Biochem., 70, 2420-2428.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA 1 mM, 1.2fold activation Lysobacter sp.
NEM 1 mM, 1.4fold activation Lysobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide 100 mM, 98% inhibition Lysobacter sp.
Hg2+ 1 mM, complete inhibition Lysobacter sp.
Monoiodoacetic acid 1 mM, 10% inhibition Lysobacter sp.
N-bromosuccinimide 1 mM, 99% inhibition Lysobacter sp.
o-phenanthroline 1 mM, 75% inhibition Lysobacter sp.
p-chloromercuribenzoate 0.01 mM, 20% inhibition Lysobacter sp.
PMSF 1 mM, 89% inhibition Lysobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 1 mM, 1.3fold activation Lysobacter sp.
Ca2+ 1 mM, 1.3fold activation Lysobacter sp.
Mg2+ 1 mM, 1.3fold activation Lysobacter sp.
Zn2+ 1 mM, 1.3fold activation Lysobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
x * 41000, SDS-PAGE Lysobacter sp.
41241
-
x * 41241, calculated from sequuence Lysobacter sp.

Organism

Organism UniProt Comment Textmining
Lysobacter sp. Q25C15
-
-
Lysobacter sp. IB-9374 Q25C15
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lysobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
Lysobacter sp.
-

Storage Stability

Storage Stability Organism
4°C, pH 6.5, 24 h , stable Lysobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
Lysobacter sp. cellobiose + ?
-
?
carboxymethyl cellulose + H2O
-
Lysobacter sp. IB-9374 cellobiose + ?
-
?
cellohexaose + H2O major product is cellotriose, minor products are cellobiose and cellotetraose Lysobacter sp. cellotriose + cellotetraose + cellobiose
-
?
cellohexaose + H2O major product is cellotriose, minor products are cellobiose and cellotetraose Lysobacter sp. IB-9374 cellotriose + cellotetraose + cellobiose
-
?
additional information the enzyme also shows chitosanase activity. Colloidal chitosan, chitosan and glycol chitosan are hydrolyzed by Cel8A at 15-40% the activity of cellulose Lysobacter sp. ?
-
?
additional information the enzyme also shows chitosanase activity. Colloidal chitosan, chitosan and glycol chitosan are hydrolyzed by Cel8A at 15-40% the activity of cellulose Lysobacter sp. IB-9374 ?
-
?

Subunits

Subunits Comment Organism
? x * 41000, SDS-PAGE Lysobacter sp.
? x * 41241, calculated from sequuence Lysobacter sp.

Synonyms

Synonyms Comment Organism
Cel8A
-
Lysobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Lysobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 20°C: about 55% of maximal activity, 60°C: about 60% of maximal activity Lysobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable up to Lysobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
cellulase activity Lysobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5 pH 4.5: about 85% of maximal activity, pH 6.5: about 40% of maximal activity Lysobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 24 h, 4°C, more than 80% of original activity is retained Lysobacter sp.
6.5
-
24 h, 4°C, most stable at Lysobacter sp.