Any feedback?
Literature summary for 3.2.1.4 extracted from
- Purification and characterization of a bifunctional enzyme with chitosanase and cellulase activity from commercial cellulase (2006), Biochem. Eng. J., 30, 82-87.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Trichoderma viride |  |
| Hg2+ | - |
Trichoderma viride | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-value is 0.1 mg/ml glucose for hydrolysis of carboxymethyl cellulose | Trichoderma viride |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
gel filtration | Trichoderma viride |
| 66000 | - |
1 * 66000, SDS-PAGE | Trichoderma viride |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma viride | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trichoderma viride |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.258 | - |
- |
Trichoderma viride |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl cellulose + H2O | - |
Trichoderma viride | ? | - |
? | |
| additional information | the hydrolase exhibited chitosanase activity and cellulase activity | Trichoderma viride | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 66000, SDS-PAGE | Trichoderma viride |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Trichoderma viride |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | 30 min to 2 h, stable | Trichoderma viride |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
hydrolysis of carboxymethyl cellulose | Trichoderma viride |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | 30°C, 24 h, stable | Trichoderma viride |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
