Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CH3COONa | 1.6 M, 4.45fold activation | Salipaludibacillus agaradhaerens | |
NH4Cl | 0.2 M, 3fold activation | Salipaludibacillus agaradhaerens | |
NH4NO3 | 0.05 M, 2.8fold activation | Salipaludibacillus agaradhaerens |
General Stability | Organism |
---|---|
enzyme is stable to incubation with hydrogen peroxide | Salipaludibacillus agaradhaerens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 2 mM, 67% inhibition | Salipaludibacillus agaradhaerens | |
Hg2+ | 2 mM, 20% inhibition | Salipaludibacillus agaradhaerens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 2 mM, 1.5fold activation | Salipaludibacillus agaradhaerens | |
KCl | 0.8 M, 4.23fold activation | Salipaludibacillus agaradhaerens | |
LiCl | 0.05 M, 1.9fold activation | Salipaludibacillus agaradhaerens | |
Mg2+ | 2 mM, 1.48fold activation | Salipaludibacillus agaradhaerens | |
Na2SO4 | 0.5 M, 4.43fold activation | Salipaludibacillus agaradhaerens | |
NaBr | 0.5 M, 3.84fold activation | Salipaludibacillus agaradhaerens | |
NaCl | the enzyme activity increass around 4fold by the addition of 0.2-2.0 M NaCl in 0.1 M glycine-NaOH buffer (pH 9.0) | Salipaludibacillus agaradhaerens | |
NaNO3 | 0.5 M, 4fold activation | Salipaludibacillus agaradhaerens | |
Sr2+ | 2 mM, 1.45fold activation | Salipaludibacillus agaradhaerens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, SDS-PAGE | Salipaludibacillus agaradhaerens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salipaludibacillus agaradhaerens | - |
JAM-KU023 | - |
Salipaludibacillus agaradhaerens JAM-KU023 | - |
JAM-KU023 | - |
Purification (Comment) | Organism |
---|---|
- |
Salipaludibacillus agaradhaerens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Salipaludibacillus agaradhaerens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
avicel + H2O | 33% of the activity with carboxymethyl cellulose | Salipaludibacillus agaradhaerens | ? | - |
? | |
avicel + H2O | 33% of the activity with carboxymethyl cellulose | Salipaludibacillus agaradhaerens JAM-KU023 | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Salipaludibacillus agaradhaerens | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Salipaludibacillus agaradhaerens JAM-KU023 | ? | - |
? | |
lichenan + H2O | 380% of the activity with carboxymethyl cellulose | Salipaludibacillus agaradhaerens | ? | - |
? | |
lichenan + H2O | 380% of the activity with carboxymethyl cellulose | Salipaludibacillus agaradhaerens JAM-KU023 | ? | - |
? | |
additional information | the enzyme does not degrade curdran, xylan, laminarin and pectin (0.5% each) at all | Salipaludibacillus agaradhaerens | ? | - |
? | |
additional information | the enzyme does not degrade curdran, xylan, laminarin and pectin (0.5% each) at all | Salipaludibacillus agaradhaerens JAM-KU023 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Salipaludibacillus agaradhaerens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
optimum without NaCl. Enzyme activity increases approximately 6fold at 60°C with 0.2 M NaCl compared to that at 50°C without NaCl in 0.1 M glycine-NaOH buffer (pH 9.0) | Salipaludibacillus agaradhaerens |
60 | - |
optimum with 0.2 M NaCl. Enzyme activity increases approximately 6fold at 60°C with 0.2 M NaCl compared to that at 50°C without NaCl in 0.1 M glycine-NaOH buffer (pH 9.0) | Salipaludibacillus agaradhaerens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.4 | - |
carboxymethyl cellulose | in presence of 25 mM | Salipaludibacillus agaradhaerens | |
84.4 | - |
carboxymethyl cellulose | in presence of 250 mM | Salipaludibacillus agaradhaerens | |
190.1 | - |
carboxymethyl cellulose | in presence of 25 mM | Salipaludibacillus agaradhaerens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | optimum without NaCl | Salipaludibacillus agaradhaerens |
7 | 9.4 | optimum with 0.2 M NaC | Salipaludibacillus agaradhaerens |